第三十六卷(2007)
44.雞隻藾菢性與性染色體上的泌乳素接受體基因之關聯性探討
林德育(1) 黃鈺嘉(1) 陳若菁(1) 鍾秀枝(1) 黃祥吉(1) 賴永裕(1) 張秀鑾(2) 吳明哲(1)
(1)行政院農業委員會畜產試驗所 (2)國立屏東科技大學
藾菢性是造成雞隻產蛋數低落的重要因素之一。本試驗利用畜試土雞近親品系9與來亨雞進行正反雜交產生雜交一代雞隻，雜交一代雞隻進行全同胞配種產生雜交二代雞隻，以建立參考族群。泌乳素接受體基因被定位於性染色體(Z)上。採集雞隻血樣進行泌乳素接受體基因(PRLR)基因型檢測，並結合母雞藾菢記錄進行關聯性分析。畜試土雞近親品系9 雞隻PRLR基因型頻率PP、PR及RR分別為42.4%、42.4%及15.2%，而來亨雞皆為PP基因型，顯示在畜試土雞近親品系9與來亨雞品系P間的PRLR基因型頻率存在顯著的關聯性 (P<0.001)。雜交二代母雞有80%無藾菢行為表現，藾菢天數達3天以上的母雞平均藾菢天數為19.6天。將無藾菢行為的母雞當作無藾菢性組，藾菢天數達3天以上的母雞則為有藾菢性組，分析PRLR基因型與有無藾菢性組間亦存在顯著的關聯性(P<0.01)。
關鍵語：雞、藾菢性、泌乳素接受體基因
ASSOCIATION STUDY OF CHICKEN BROODINESS AND PROLACTIN RECEPTOR GENE
D.Y. Lin(1), Y.C. Huang(1), J.C. Chen(1), H.C. Chung(1) , C.H. Huang(1) Y.Y. Lai(1) H L. Chang(2) and M C. Wu(1)
(1)Livestock Research Institute(LRI), Council of Agriculture
(2)National Pingtung University of Science and Technology
Chicken broodiness is considered as one of the major factors that decrease the potential of egg production. In order to set up a F2 design reference family for mapping of broodiness, the F1 hybrid progenies was produced by reciprocal crosses between LRI native chicken inbred line 9( LRI-L9) and Leghorn, and F2 progenies was produced by fullsib mating of F1. Prolactin receptor gene(PRLR) was on sex chromosome Z. Records of genotyping of DNA extracted from blood merged with broody records was used to explore the association between broodiness and genotype. PRLR of PCR-SSCP has three genotypes. The genotype frequency of PP, PR and RR in LRI-L9 were 42.4%, 42.4% and 15.2%, respectively. But, all leghorns were PP type in the collected samples. The G0 data showed an strong association between breed and genotype (P<0.001). Eighty percent of hens of F2 don’t express broody behavior. The average broody days of broody hens, with more than three days continually broody records, were 19.6 days. The link between PRLR genotypes and broodiness was also significant in the F2 data set (P<0.01).
Key Words: Chicken, Broodiness, Prolactin receptor gene
  
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