R. B. Liaw, W. Y. Huang, Y. S. Cheng and M. C. Wu Livestock Research Institute, Council of Agriculture, Executive Yuan
The purpose of this study is to construct large-insert libraries to preserve valuable genes and to provide for the future genomic studies. In this study, the plateform of large-insert DNA separation and separation and purification of large partially digested DNA from Brown Tsaiya was primarily established. At the same time, the core technology of BAC library construction was set up. The average insert sizes were 34.0 and 48.9 kb, respectively, for two constructed genomic libraries. The total number of preserved clones reached to 110,000 and the genome coverage of the entire library was 3.69-fold.