第四十三卷(2014)
努比亞種羊黏多醣症與肉質相關基因之基因型分析
顏念慈(1)、陳若菁(1)、陳芃諭(2)、賴永裕(1)、林德育(1)、吳昇陽(1)、陳佳萱(1)、吳明哲(1)、朱賢斌(1)、 張秀鑾(2)
(1)行政院農業委員會畜產試驗所 (2)國立屏東科技大學動物科學與畜產系
本試驗之目的為利用PCR-RFLP 或PCR-SSCP方法，對來自東台灣的種羊場28頭與南部的種羊場75頭努比亞種羊，進行黏多醣症(Mucopolysaccharidosis,G6S) 及肉質相關基因腦下垂體特異性轉錄因子(pituitary transcription factor, POU1F1) 與鈣蛋白酶抑制蛋白 (Calpastatin, CAST) 基因之基因型分析。初步結果，G6S基因型有正常型AA與雜合型AB兩種，AB頻率為0.10 (1/103)，而南部的種羊場努比亞種羊皆為正常型。POU1F1是依照NCBI的DQ826413 DNA序列設計一對引子，去PCR增殖努比亞種羊DNA，得到481 bp片斷，再用AluI 限制酶切割，可分出CC 與TC 基因型，其頻率分別為0.79 與0.21。而依照Zhou et al. (2008) 設計之一對引子增殖努比亞種羊DNA，得到全長253或254 bp之CAST片段，再進行SSCP分析，可分出A, B及C態樣，其頻率分別為0.77、0.13及0.10。以上結果可供努比亞種羊場進行G6S-AA型培育與未來努比亞種羊肉質基因選種之參考。
關鍵語：努比亞種羊、肉質基因、黏多醣症
GENOTYPING OF MUCOPOLYSARCHARIDOSIS AND GENES IN ASSOCIATION WITH MEAT QUALITY FOR NUBIAN GOAT
N. T. Yen(1), J. C. Chen(1), P. Y. Chen(2), Y. Y. Lai(1), D. Y. Lin(1), S. Y. Wu(1), C. H. Chen(1), M. C. Wu(1), H. P. Chu(1) and H. L. Chang(2)
(1)Livestock Research Institute, Council of Agriculture, Executive Yuan;
(2) Department of Animal Science, National Pingtung University of Science and Technology
The aim of this study was to detect the genotypes of Mucopolysaccharidosis and genes in association with meat quality for Nubian goat. Totally 103 genomic DNA samples were collected from two goat farms located in eastern Taiwan and southern Taiwan for defining the genotype of (Mucopolysaccharidosis, G6S), (pituitary transcription factor, POU1F1), and (Calpastatin, CAST) genes by PCR-RFLP or by PCR-SSCP method. Genotyping frequencies of each gene for Nubian goat were analyzed. The preliminary results, there were two G6S genotypes, normal type AA and heterozygous type AB. The frequency for AB type was 0.10 (1/103), and all 75 Nubian goats were normal from farm located in southern Taiwan. According to DQ826413 DNA sequence of NCBI, we designed a pair primer for POU1F1 gene to amplify Nubian DNA by PCR, and got the 481 bp products, then digested with AluI restriction enzyme. The POU1F1 genotype frequencies of CC , and CT were 0.79 and 0.21, respectively. And we used a pair primer designed by Zhou et al. (2007) for CAST gene to amplify Nubian DNA by PCR, and got the 253 or 254 bp products, then genotyped by SSCP analysis. The SSCP analysis of CAST gene constituted three different genotypes, A, B and C. The CAST genotype frequencies of A, B and C were 0.77, 0.13 and 0.10, respectively. The results are potentially useful for Nubian goat farmer to breed G6S-AA type goat and to get good meat quality by gene selection in the future.
Key Words: Nubean goat, Meat quality gene, Mucopolysaccharidosis
  
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