第四十三卷(2014)
種豬粒線體DNA D環區序列之分析
廖仁寶 陳若菁 陳玟岑 王玉雪 吳明哲
行政院農業委員會畜產試驗所
本研究之目的在於探討三個品種選拔指數不小於110之種豬粒線體部分D環區序列之變異，藉以瞭解品種間與品種內之差異。試驗樣品總計有190頭種豬之粒線體DNA，包括64頭杜洛克豬、63頭藍瑞斯豬及63頭約克夏豬。設計D環區特定引子對，用以PCR增幅特定片段，產物純化後進行DNA序列解析，所有DNA序列經過多重比對分析後，在557 bp的DNA序列範圍內發現了26個變異點，大部分之變異型式為T/C（17點），其餘有A/G（7點），A/T (1點)及C缺失(1點)。在三個豬種裡，約克夏豬具有最多的變異點25個，其次為杜洛克豬19點，最少為藍瑞斯豬13點。在類緣演化分析中顯示，某些群叢包含了兩個品種以上的豬隻樣品，由此可再進一步探討可能的原因。
關鍵語：粒線體DNA D環區、變異點、種豬
SEQUENCE VARIATION ANALYSIS OF MITOCHONDRIAL DNA D-LOOP REGION OF BREEDING PIGS
R. B. Liaw, J. C. Chen, W. C. Chen, Y. S. Wang and M. C. Wu
Livestock Research Institute, Council of Agriculture, Executive Yuan
The aim of this study was to investigate the sequence variation of mitochondrial DNA D-loop region of and to discover the difference between and within three breeds of breeding pigs with a selection index no less than 110. A total of 190 mtDNA samples from 64 heads of Duroc pigs, 63 heads of Landrace pigs, and 63 heads of Yorkshire pigs were tested. The PCR products were amplified with mtDNA D-loop region specific primers and were purified by a purification kit. After DNA sequencing and multiple alignment of all sequences, 26 variation sites were found in 557-bp DNA segment. Most of the variation type was T/C pattern (17 points), followed by A/G pattern (7 points), A/T pattern (1 point), and C deletion (1 point). Among three breeds of pigs, Yorkshire, Duroc, and Landrace possessed 25, 19, and 13 variation sites, respectively. Based on the phylogenetic analysis, more than two breeds of pigs were found on the same cluster. Therefore, it deserves further study to find possible causes.
Key Words: Mitochondrial DNA D-loop, Variation site, Breeding pig
  
|
|
|
