GENETIC DIVERSITY ANALYSIS OF HIGH EGG PRODUCTION LINE CM NATIVE CHICKEN IN LRI-COA BY MICROSATELLITE MARKERS
D. Y. Lin(1), S. J. Tzeng(2), Y. F. Lin(1), H. L. Lin(1), H. L. Chang(3) and M. C. Wu(1)
(1)Livestock Research Institute(LRI), Council of Agriculture (2)Chung Hwa University of Medical Technology (3)National Pingtung University of Science and Technology
LRI high egg production line CM native chicken, which was selected for numbers of egg based on the F1 population crossed by the cocks of inbreeding line L12 native chicken of Council of Agriculture Livestock Research LRI and hens of commercial brown layer for six generation self-cross. To assess the genetic variability of high egg production line CM breeding populations, we use a set of 24 microsatellite markers to analyze 80 candidate bred chickens from this flock. All of the microsatellites were polymorphic. The average allelic number was 3.3, ranged from 2 to 7 per locus. The expected heterozygosity ranged from 0.013 to 0.701, and the average expected heterozygosity was 0.51±0.19 (mean ±SD). The observed heterozygosity of the population ranged from 0 to 0.738, and the average observed heterozygosity was 0.49±0.22. The estimated average polymorphic information content (PIC) was 0.44±0.18. In 24 markers, eleven markers were highly informative with polymorphism information content (PIC ≥0. 50), ten markers were reasonably informative (0.5> PIC≥ 0.25) and the other three markers were slightly informative (PIC < 0.25). These results could be provided basic molecular information for the research on the germplasm characteristics of high egg production line CM native chicken in LRI-COA.