第四十六卷(2017)
檢定站種公豬精子成熟度檢測及應用
朱巧倩(1)、王受鎔(2)、林鴻霖(2)、郭廷雍(1)、賴永裕(1)、林秀蓮(1)、吳明哲(1)
(1)行政院農業委員會畜產試驗所 (2)財團法人中央畜產會
本計畫目的為加倍公豬高飼料效率與產精遺傳同步選拔效率，藉由精子體能分析儀測定年青種公豬之精液濃度及精子粒線體完整度，以評估年青公豬產精能力與成熟度，期提早應用優質的高飼料效率種公豬於種豬繁殖及肉豬生產上，加速優質基因之擴散利用。測定之年青種公豬為財團法人中央畜產會種豬性能檢定站201607期、201609期、201610期及201611期完檢之杜洛克、藍瑞斯及約克夏等3個品種計257頭種公豬。種公豬於拍賣前20天採集精液，採集之新鮮精液儲存於 17℃保溫攜回實驗室測定精液濃度及同步快速測定每頭公豬精液至少5,000隻精子之粒線體完整度，作為判別年青公豬產精能力指標。檢測結果顯示，杜洛克（n=171）、藍瑞斯（n=58）及約克夏（n=28）公豬其各項分析項目之結果以平均值±標準偏差表示，精液濃度分別為3.51 ± 1.01、4.01 ± 0.99及3.09 ± 1.06億/毫升；精子粒線體完整度分別為57.2 ± 19.5、52.2 ± 27.8及54.0± 28.5%。
關鍵語：種豬、精子、粒線體完整性 級
Application of flow cytometer to evaluation of sperm maturity in breeding pig of the pig performance testing station
C. C. Chu(1), S. R. Wang(2), H. L. Lin(2), T. Y. Kuo(1), Y. Y. Lai(1), H. L. Lin(1), and M. C. Wu(1)
(1)Livestock Research Institute, Council of Agriculture, Executive Yuan (2)National Animal Industry Foundation
The objective of this study is conducted to measure the sperm concentration and mitochondrial integrity by using flow cytometer to evaluate the semen productive ability and maturity of high feed efficiency young boar, and try to apply the elite young boar for the reproduction of breeding stock and the production of hog early. Finished test boars from 3 breeds (Duroc, Landrace and Yorkshire) in class 201607, 201609, 201610, and 201611 of the Pig Performance Testing Station of National Animal Industry Foundation were used at this project. We collected the semen 20 days before the auction and stored at 17℃. The collected semen were immediately analyzed the sperm concentration and mitochondrial integrity at least 5,000 sperm each semen to assess the semen productive ability of young boar. The results showed that the sperm concentration and mitochondrial integrity of the young boars from Duroc (n=171), Landrance (n=58) and Yorkshire (n=28) were 351 ± 101 (106/ml), 401 ± 99 (106/ml), 309 ± 106 (106/ml) and 57.2 ± 19.5 (%), 52.2 ± 27.8 (%), 54.0 ± 28.5 (%), respectively.
Key Words: Breeding pig, Sperm, Mitochondrial Integrity
  
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