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分頁目錄
1. 檢定站種公豬精子成熟度檢測及應用
2. 生長檢定豬與體型比賽豬之選種性狀差異分析
3. 臺灣豐和黑毛烏骨雞之微衛星遺傳標記多態性分析
4. 豐輝下營紅牌土雞之微衛星遺傳標記多態性分析
5. 民間種雞場家禽白血病J病毒的監測
6. 畜試紅公豬各世代生長性能
7. POU1f1基因型對努比亞山羊肉質性能表現之分析
8. 異地保種小型豬族群基因多樣性分析
9. 乳牛第11凝血因子缺失症基因型頻率分析
10. 活性污泥多源酯酶基因次選殖與表現分析
第四十六卷(2017)
民間種雞場家禽白血病J病毒的監測

林德育(1) 曾淑貞(2) 賴永裕(1) 邢湘琳(1) 張秀鑾(3) 吳明哲(1)
(1)行政院農業委員會畜產試驗所 (2)中華醫事科技大學 (3)國立屏東科技大學

家禽白血病(avian leucosis, AL)是由家禽白血病病毒(avian leucosis virus, ALV)引起,其中的 J 亞群(subgroup J ALV;ALV-J)於 1988 年出現,造成養雞業者的嚴重損失。為了解民間種雞場種雞群是否感染家禽白血病 J 病毒,從 2011 年至 2017 年逐批監測進駐行政院農業委員會畜產試驗所創新育成中心的種雞場選育族群候選種雞是否感染家禽白血病 J 病毒。採集以含抗凝劑 EDTA-K3 隻採血器採集雞隻翼靜脈 2 毫升全血,低溫寄送國立臺灣大學獸醫專業學院禽病學研究室進行雞白血病檢測,每批採集 23 隻候選種雞,全血抽取 DNA 進行 PCR(primer H5/H7)檢測家禽白血病 J 病毒。監測 4 家民間種雞場種,4 個雞種。包括 16 批紅羽土雞共 365 隻、1批 23 隻黑羽土雞、8 批商用烏骨雞共 181 隻及 1 批 23 隻藍殼蛋黑羽烏骨雞,共計 592 隻候選種雞。檢測結果在 2011 年有1批商用烏骨雞 23 隻送檢樣品中有5隻檢出家禽白血病 J 病毒,並已將該陽性雞隻立即淘汰,而在其他所有送檢樣品皆呈陰性反應。因此,繼續監測種雞群是否潛藏家禽白血病 J 病毒仍有其必要性。

關鍵語:家禽白血病、家禽白血病J病毒、雞、監測


Avian leucosis J-virus monitoring in private chicken breeder farms

D. Y. Lin(1), S. J. Tzeng(2), Y. Y. Lai(1), S. L. Hsing(1), H. L. Chang(3) and M. C. Wu(1)
(1)Livestock Research Institute(LRI), Council of Agriculture (2)Chung Hwa University of Medical Technology (3)National Pingtung University of Science and Technology

Avian leucosis is caused by Avian leucosis viruses (ALVs). are prevalent in the poultry industry worldwide and cause severe economic losses. The subgroup J of ALV (ALV-J) has emerged as an important pathogen of meat-type chickens since 1989 and causes serious economic losses in commercial poultry industry. In order to monitor ALV-J disease in the private selection country chicken flocks of those stationed in the Innovation Incubation Center of LRI-COA, we collected candidate bred chicken blood samples by batch, 23 samples per batch, used the blood collection with anticoagulant EDTA-K3 from 2011to 2017. Each bird was collected 2 ml of whole blood from wing vein. Blood samples were sent to Lab of Poultry Diseases in School of Veterinary Medicine National Taiwan University for ALV-J detection. Four breeding farms including four country chicken breeds were monitored in this program. Totally 592 candidate bred chicken blood samples were detected including 365 Red Feathered country chicken from 16 batches, 23 Black Feathered country chickens of one batch, 181 Commercial Silky chickens from 8 batches and 23 Black Silky chickens for one batch. Only five samples of one batch were detected ALV-J positive in one farm. The positive birds were eliminate immediately. Others of all were ALV-J negative. Thus, it is necessary to continuously monitor the chicken breeding flocks harbored ALV-J seriously.

Key Words: Avian leucosis, ALV-J, Chicken, Monitoring



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