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STUDY POLYMORPHISM OF RANDOM AMPLIFIED POLYMORPHIC DNA IN WHITE ROMAN GOOSE
D. Y. Lin(1) , J. J. Liu Tai (1), J. C. Chen(1) , L. T. Yeh(1) , H. L. Chang(1) and C. Tai(2)
(1)Taiwan Livestock Research Institute, Council of Agriculture (2)Institute of Biotechnology, National Cheng Kung University
The purpose of this study was to explore the association between production performance and RAPD (random amplified polymorphic DNA) polymorphism of White Roman Goose. Blood samples of 48 geese were collected based on mature body weight(male) and egg production performance(female). Twelve best or worst geese were assorted into each low body weight(LW), high body weight(HW), low egg production(LE) and high egg production(HE) groups. Individual genomic DNA was extracted from blood sample and detected concentration for DNA template. There were 328 kinds of a single, short (10 bp) oligonucleotide for RAPD tests. After electrophoresis in 1.5¢M agarose gels and stained with ethidium bromide, prints of the PCR amplified products were scanned and analyzed by Gelcompar software. The results showed 85% of these primers got PCR products, and 41% got polymorphic PCR products. The primers, AC09(890bp), AE03(690bp), AE06(920bp), AG10(1350bp, 1370bp) and AL13(1288bp), produced distinct PCR products for different genders and polymorphic patterns of AD06(720bp), AD14(700bp), AH13(2400bp) and AJ15(510bp) were not independent between high and low egg production groups.
Key Words: White Roman Goose, Random amplified polymorphic DNA (RAPD), Polymorphism.
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