第四十卷(2011)
應用精子體能分析儀評估紅羽土雞精液性狀之研究
邢湘琳 林秀蓮 林德育 賴永裕 吳明哲
行政院農業委員會畜產試驗所
為建立完善之精液性狀評估系統與提昇種土雞生產效益，本試驗利用畜試所輔導進駐育成中心之種雞場所選育之紅羽土雞種雞群進行精液性狀檢測，評估種雞產精能力及精液品質。檢測項目包含：存活率、頭帽及精子膜完整率、粒線體完整率、鈣離子濃度及細菌數。某民間種雞場送檢12隻約25周齡之紅羽土雞精液，經精子體能分析儀檢測結果為精子存活率其排名前50%平均值與最佳值各為94.90%及98.21%、活精且頭帽完整率各為98.33%及99.06%、線粒體膜完整性各為99.12%及100%、鈣離子濃度各為257.38AU及188.82AU、細菌數各為2.4 x 105個/ml及5 x 104個/ml。試驗結果經分析後可得排名前50%平均值與最佳值，分別可作為該項之選留標準及性狀最優者。檢測結果進一步可評估其產精能力，並應用於種雞選留時。
關鍵語：紅羽土雞、精液性狀
APPLYING EASYCYTE TO ESTIMATE QUALITY OF RED FEATHER NATIVE CHICKEN SEMEN
S. L. Hsing, H. L. Lin, D. Y. Lin, Y. Y. Lai, and M. C. Wu
Livestock Research Institute (LRI), Council of Agriculture
To establish a comprehensive semen quality evaluation system and to enhance the efficiency of breeding and productivity of native chicken, this study use semen samples of native Taiwan red feather chicken provided by the breeding farm under the program of the Innovation Incubator Center of Livestock Research Institute to develop a evaluation system on quality and productivity of Taiwanese rooster semen. The objective of this study mainly examines semen: viability (survival rate), acrosome and sperm membrane integrity, mitochondria integrity (mitopotential), calcium level and bacterial count. The semen of 12 red feather native chickens about 25 weeks old from the native chicken breeding farm was analyzed by EASYCYTE. We found: the 50th percentile average and maximum viability of our sample are 94.90% and 98.21% respectively. Similarly, the corresponding 50th percentile average and maximum value of intact acrosome and sperm membrane of the sample are 98.33% and 99.06% respectively; the intact mitochondria are 99.12% and 100% correspondingly; with calcium level of 257.38AU and 188.82AU and the bacterial count of 2.4 x 105 /mL and 5 x 104 /mL respectively. The 50th percentile average and maximum value of the sample can be used as the selection criterion for roosters breeding. The findings can further be used to evaluate and estimated sperm productivity and performance and can be applied to select breeding roosters.
Key Words: Red feather native chicken, Quality of semen
  
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