第四十卷(2011)
保種華鵝基因多樣性分析
廖仁寶 陳若菁 林秀蓮 蕭智彰 賈玉祥 吳明哲
行政院農業委員會畜產試驗所
本研究之目的在於利用微衛星型遺傳標記探討保種褐色與白色華鵝之基因多樣性。以10組微衛星型遺傳標記分析畜產試驗所彰化種畜繁殖場106隻褐色華鵝與118隻白色華鵝之基因多樣性，其交替基因數、觀測異質度(Ho)、期望異質度(He)及多態性訊息量(PIC)範圍分別為1–6 vs. 2–5、0–0.642 vs. 0–0.712、0–0.663 vs. 0.014–0.683及0–0.598 vs. 0.014–0.625，而其平均值則分別為3.0 vs. 3.5、0.332 vs. 0.237、0.401 vs. 0.331及0.342 vs. 0.291，可得知此群華鵝的基因多樣性亦僅具中度多態性資訊。由本研究所得之基因多樣性分析參數，將可提供當作未來保種族群繁衍管理之參考依據。
關鍵詞：保種華鵝、微衛星型遺傳標記、基因多樣性。
GENETIC DIVERSITY OF CONSERVED CHINESE GEESE EVALUATING BY MICROSATELLITE MARKERS
R. B. Liaw, R. J. Chen, H. L. Lin, C. C. Hsiao, Y. S. Jia and M. C. Wu
Livestock Research Institute, Council of Agriculture, Executive Yuan
The objective of this study was to investigate the genetic diversity of the conservative population of Brown and White Chinese geese using microsatellite genotyping. A total of ten microsatellite markers were used to genotype 106 Brown Chinese geese and 118 White Chinese geese of Changhua propagation station, Livestock Research Institute. The values for allele number, observed heterozygosity (Ho), expected heterozygosity (He), and polymorphism information content (PIC) of both lines of geese among all loci were in the range of 1–6 vs. 2–5, 0–0.642 vs. 0–0.712, 0–0.663 vs. 0.014–0.683 and 0–0.598 vs. 0.014–0.625, respectively. The mean values for allele number, Ho, He, and PIC were 3.0 vs. 3.5, 0.332 vs. 0.237, 0.401 vs. 0.331 and 0.342 vs. 0.291, respectively. The result also indicates that the genetic diversities of both Chinese geese populations are moderate. In the future, the parameters of genetic diversity can be referred to the reproduction administration of conservative populations.
Key Words: Conserved Chinese Geese, Microsatellite marker, Genetic diversity.
  
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