第四十卷(2011)
恆春黑羊粒線體D-loop分析
陳佳萱(1) (2) 王勝德(3) 張慕慈(2) 莊壁華(4) 楊深玄(3) 李光復(3) 吳明哲(1) 黃政齊(3)
(1)行政院農業委員會畜產試驗所 (2)國立中興大學動物科學系 (3)行政院農業委員會畜產試驗所恆春分所 (4)行政院農業委員會畜產試驗所花蓮種畜繁殖場
本試驗目的旨在利用粒線體D-loop區域序列分析恆春黑羊與其它品種山羊之親緣關係。試驗採集恆春分所之恆春黑羊、黑色波爾、台灣黑山羊恆春品系與民間場之波爾、努比亞山羊共21頭血液樣品並萃取其DNA，依據山羊mtDNA D-loop區域序列設計專一引子對，進行山羊血樣DNA之PCR反應，純化產物後進行序列解析。另外自NCBI基因庫下載現有山羊品種mtDNA D-loop序列，包括Yudong white、Nanjiang、Qianbei grey、Guizhou white、Lezhi black、Yingshan black、China Boer及Ammotragus Lervia山羊供作外群使用。利用全部mtDNA D-loop區域序列以鄰接法建構山羊演化關係圖，以暸解不同山羊品種間之親緣關係。演化樹分析結果將受測羊群分為五群，第一群包括恆春黑羊、台灣黑山羊恆春品系、黑色波爾山羊與努比亞山羊，第二群為Yudong white山羊，第三群為Guizhou white山羊、第四群為Nanjiang及Qianbei grey山羊，第五群包括China Boer、Lezhi black、Yingshan black與波爾山羊。綜合上述結果得知，恆春黑羊與台灣黑山羊恆春品系、努比亞及黑色波爾山羊親緣關係甚高。
關鍵語：D-loop、粒線體DNA、山羊
ANALYSIS OF MITOCHONDRIAL DNA D-LOOP REGION IN HENGCHUN BLACK GOATS
C. H. Chen(1) (2), S. D. Wang(3), M. T. Chang(2), P. H. Chuang(4), S. S. Yang(3), G. F. Lee(3), M. C. Wu(1) and J. C. Haung(3)
(1) Livestock Research Institute, Council of Agriculture (2) Department of Animal Science, National Chung Hsing University (3) Hengchun Branch, COA-LRI (4) Hualien Animal Propagation Station, COA-LRI
The purpose of this study was to investigate the phylogenetic relationships among Hengchun black goats and other goats using mitochondrial DNA (mtDNA) D-loop sequences. Total DNA was extracted from blood samples of Hengchun black goat, solid black Boer goat, Taiwan black goat, Boer goat, and Nubian goat. The primer pair specific to mtDNA D-loop region of goat was designed and used to amplify 21 goat DNAs. After purification, the PCR products were sequenced by an automatic DNA sequencer. We also downloaded mtDNA D-loop sequences of Yudong white goat, Nanjiang goat, Qianbei grey goat, Guizhou white goat, Lezhi black goat, Yingshan black goat, China Boer goat and Ammotragus Lervia goat (out group) from NCBI GenBank. All the sequences of D-loop region were used to construct phylogenetic trees using Neighbor-joining methods. The results showed that all analyzed goats were divided into five groups using phylogenetic relationship analysis, of which Hengchun black goat, Taiwan black goat, solid black Boer goat and Nubian goat was in the first group; Yudong white goat was in the second group; Guizhou white goat was in the third group; Nanjiang and Qianbei grey goats were in the fourth group; Lezhi black goat, Yingshan black goat, China Boer goat, and Boer goat was in the fifth group. Based on the above results, Hengchun black goat has a phylogenetic indication closely relating to Taiwan black goat, Nubian goat and solid black Boer goat.
Key Words: D-loop, Goat, Mitochondrial DNA
  
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