GENETIC DIVERSITY ANALYSIS OF FORMOSAN SAMBAR IN LRI-COA BY MICROSATELLITE MARKERS
D. Y. Lin, S. R. Kang, Y. Y. Lai, H. H. Lin, C. H. Wang and M. C. Wu
Livestock Research Institute(LRI), Council of Agriculture
Formosan Sambar is a one of sambar deer subspecies exclusively found in Taiwan. It is also the largest native herbivore of the island and has been raised as farm animal more than one hundred years. The Deer population was established in Kaohsiung Animal Propagation Station since 1984. In order to construct the information of genetic diversity of this population, we use a set of 11 microsatellite markers to analyze 126 individuals from this herd. All of the microsatellites were polymorphic with average allelic number 7.5, ranged from 3 to 17 per locus. There were 83 alleles detected in total. The observed heterozygosity of the population ranged from 0.04 to 0.63, and the average observed heterozygosity was 0.33±0.18(mean±SD). The expected heterozygosity ranged from 0.12 to 0.78, and the average expected heterozygosity was 0.64±0.20. The estimated average polymorphic information content (PIC) was 0.60± 020. Except NVHRT73 marker was slightly informative (PIC < 0.25) and two markers (BL42 and BL203) were reasonably informative (0.50 > PIC > 0.25), others of the microsatellite markers were highly informative highly informative (PIC > 0.50). Our result indicated that the geneticists have paid more attention in keeping genetic diversity of the Formosan Sambar herd.