第四十二卷(2013)
畜產試驗所台灣水鹿微衛星遺傳標記多樣性分析
林德育 康獻仁 賴永裕 林信宏 王治華 吳明哲
行政院農業委員會畜產試驗所
台灣水鹿是台灣最大型的野生草食動物，並被馴養為畜產動物已超過一百年。行政院農業委員會畜產試驗所高雄種畜繁殖場自1984年起開始建立台灣水鹿族群，為建立該族群之遺傳多樣性資訊，本試驗利用11組微衛星標記分析該族群126頭台灣水鹿個體之DNA，所有11組微衛星標記皆有多態型的基因型，共檢測到83個alleles，平均每個基因座具有7.5個對偶基因(3~17個alleles)，其觀測異質度介於0.04到0.63，平均為0.33 ± 0.18，期望異質度介於0.12到0.78，平均為0.64 ± 0.20，而多態性訊息含量平均為0.60 ± 0.20。在本試驗選用的11組微衛星標記組皆有多態型的基因型，除NVHRT73微衞星標記之多態性訊息含量為低多態性資訊 (PIC < 0.25)，BL42 and BL203兩組微衞星標記為中多態性資訊(0.25
關鍵語：台灣水鹿、遺傳多樣性、微衞星標記
GENETIC DIVERSITY ANALYSIS OF FORMOSAN SAMBAR IN LRI-COA BY MICROSATELLITE MARKERS
D. Y. Lin, S. R. Kang, Y. Y. Lai, H. H. Lin, C. H. Wang and M. C. Wu
Livestock Research Institute(LRI), Council of Agriculture
Formosan Sambar is a one of sambar deer subspecies exclusively found in Taiwan. It is also the largest native herbivore of the island and has been raised as farm animal more than one hundred years. The Deer population was established in Kaohsiung Animal Propagation Station since 1984. In order to construct the information of genetic diversity of this population, we use a set of 11 microsatellite markers to analyze 126 individuals from this herd. All of the microsatellites were polymorphic with average allelic number 7.5, ranged from 3 to 17 per locus. There were 83 alleles detected in total. The observed heterozygosity of the population ranged from 0.04 to 0.63, and the average observed heterozygosity was 0.33±0.18(mean±SD). The expected heterozygosity ranged from 0.12 to 0.78, and the average expected heterozygosity was 0.64±0.20. The estimated average polymorphic information content (PIC) was 0.60± 020. Except NVHRT73 marker was slightly informative (PIC < 0.25) and two markers (BL42 and BL203) were reasonably informative (0.50 > PIC > 0.25), others of the microsatellite markers were highly informative highly informative (PIC > 0.50). Our result indicated that the geneticists have paid more attention in keeping genetic diversity of the Formosan Sambar herd.
Key Words: Formosan Sambar, Genetic diversity, Microsatellite marker
  
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