GENETIC DIVERSITY ANALYSIS OF INBREEDING LINE L12 NATIVE CHICKEN IN LRI-COA BY MICROSATELLITE MARKERS
D. Y. Lin(1), S. J. Tzeng(2), Y. F. LIN(1), H. L. LIN(1), H. L. Chang(3) and M. C. Wu(1)
(1)Livestock Research Institute (LRI), Council of Agriculture (2)Chung Hwa University of Medical Technology (3)National Pingtung University of Science and Technology
In order to evaluate genetic variation of inbreeding line L12 native chicken flock in Livestock Research Institute-Council of Agriculture (LRI-COA), Executive Yuan. We use a set of 24 microsatellite markers to analyze 80 candidate bred chickens from this flock. All the microsatellites were polymorphic. The average allelic number was 2.7, ranged from 2 to 6 per locus. The expected heterozygosity ranged from 0.061 to 0.657, and the average expected heterozygosity was 0.40±0.19 (mean ±SD). The observed heterozygosity of the population ranged from 0.038 to 0.888, and the average observed heterozygosity was 0.41±0.23. The estimated average polymorphic information content (PIC) was 0.34±0.16. In 24 markers, six markers were highly informative with polymorphism information content (PIC ≥0. 50), eleven markers were reasonably informative (0.5> PIC≥ 0.25) and the other seven markers were slightly informative (PIC < 0.25). These results could be provided basic molecular information for the research on the germplasm characteristics of the population.