第四十三卷(2014)
畜試土雞近親品系L9之微衛星遺傳標記多樣性分析
林德育(1) 曾淑貞(2) 洪哲明(1) 賴永裕(1) 張秀鑾(3) 吳明哲(1)
(1)行政院農業委員會畜產試驗所 (2)中華醫事科技大學 (3)國立屏東科技大學
為評估行政院農業委員會畜產試驗所畜試土雞台畜一號近親品系L9選育族群的遺傳變異，本試驗利用FAO(2004)建議使用的23組雞微衛星標記組，以及LEI0258微衛星標記組，共24組微衛星標記組分析該族群候選種雞80隻之個體DNA。其中除MCW0216與MCW0081兩組微衛星標記所檢測的基因型在所有檢測個體皆為單型外，其它22組微衛星標記皆有多態型。共檢測到72個對偶基因，平均每個基因座具有3.0個對偶基因(1~6個)，其期望異質度介於0到0.734，平均為0.49，觀測異質度介於0到0.838，平均為0.47，而多態性訊息含量平均為0.42。在選用的24組微衛星標記組中有10組呈現高度多態性資訊(PIC ≥ 0.5)，有11組呈現中度多態性資訊(0.5> PIC ≥ 0.25)，3組呈現低度多態性資訊(PIC < 0.25)。本結果提供此選育族群遺傳變異之基本分子資訊。
關鍵語：土雞、遺傳多樣性、微衞星標記
GENETIC DIVERSITY ANALYSIS OF INBREEDING LINE L9 NATIVE CHICKEN IN LRI-COA BY MICROSATELLITE MARKERS
D. Y. Lin(1), S. J. Tzeng(2), C. M. Hung(1), Y. Y. Lai(1), H. L. Chang(3) and M. C. Wu(1)
(1)Livestock Research Institute (LRI), Council of Agriculture (2)Chung Hwa University of Medical Technology (3)National Pingtung University of Science and Technology
In order to evaluate genetic variation of inbreeding line L9 native chicken flock in Livestock Research Institute-Council of Agriculture (LRI-COA), Executive Yuan. We use a set of 24 microsatellite markers to analyze 80 candidate bred chickens from this flock. Except MCW0216 and MCW0081, all of the microsatellites were polymorphic. The average allelic number was 3.3, ranged from 1 to 6 per locus. The expected heterozygosity ranged from 0 to 0.734, and the average expected heterozygosity was 0.49±0.21 (mean ±SD). The observed heterozygosity of the population ranged from 0 to 0.838, and the average observed heterozygosity was 0.47±0.26. The estimated average polymorphic information content (PIC) was 0.42±0.19. In 24 markers, seven markers were highly informative with polymorphism information content (PIC ≥0. 50), eleven markers were reasonably informative (0.5> PIC≥ 0.25) and the other six markers were slightly informative (PIC < 0.25). These results could be provided basic molecular information for the research on the germplasm characteristics of the population.
Key Words: Native chicken, Genetic diversity, Microsatellite marker
  
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