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第四十五卷(2016)
生長性能檢定合格種豬之體型評級與XY 染色體之骨架基因關係研究
吳明哲(1) 陳美如(1) 廖仁寶(1) 賴永裕(1) 顏念慈(1) 林秀蓮(1) 蔡秀容(1) 林鴻霖(2) 王受鎔(2) 林正祥(2) 陳培梅(3) 陳中興(3)
(1)行政院農業委員會畜產試驗所 (2)中央畜產會種豬檢定站 (3)行政院農業委員會畜牧處
杜洛克(D)、藍瑞斯(L)及約克夏(Y)豬種於新化檢定站進行生長性能檢定至體重110Kg(母 100Kg)或160+7 日齡。本研究利用201101 期至201604 期等42 期檢定豬隻，總計4,713 頭血 樣，包括D 公2,482 頭、D 母201 頭、L 公1,154 頭、L 母313 頭、Y 公466 頭與Y 母97 頭。 分析微衛星標記SY11(骨架基因)在X 染色體73cM 位置交替基因型，骨架基因之交替基因間 鹼基數長短(bp)依序有161~177bp 等9 個交替基因。品種及性別差異上，使用拍賣成交頭數 最多的兩種基因型(鹼基數)來標示，在D 公是175+0 及171+0、D 母是171+4 及175+0、L 公 是171+0 及167+0、L 母是167+4 及171+0、Y 公是171+0 及169+0 與Y 母是171+0 及169+2。 不分性別，骨架基因的交替基因在D 品種以175、171 及167bp 居多；L 品種以171 及167bp 居多；Y 品種以171 及169bp 居多。每期檢定合格種豬經體型評級，取各品種各性別之體型 高大冠軍豬各一頭，總計204 頭。體型高大冠軍豬性染色體的骨架基因交替基因型在D、L、 Y 品種分別為175+0、171+0、171+0 居多，因此，應用基因條碼及體型資料作為品種內高大 體型選拔品系選育用，有利於高大型種豬經濟性狀基因標記建置及選種。
關鍵語：種豬、遺傳標記、體型評級
Association analysis of conformation contest to skeletal integrity gene on chromosome X and Y from purebred pigs under growth performance test
M. C. Wu(1), M. L. Chen(1), R. B. Liao(1), Y. Y. Lai(1), N. T. Yen(1), H. L. Lin(1), H. L. Tsai(1), H. R. Lin(2), H. L. Wang(2), C. H. Lin(2), P. M. Chen(3) and C. H. Chen(3)
(1)Livestock Research Institute, Council of Agriculture; (2)Swine Growth Performance Test Station, National Animal Industry Foundation; (3)Animal Industry Division, Council of Agriculture
Duroc (D), Yorkshire (Y) and Landrace (L) pigs were tested for growth performance at Hsinhua Station. The off-tested age was on the weight of 110kg (100kg for gilt) or by 160+7 days of age. A total of 4,713 pig blood samples was collected during the sum of 42 classes from class 201101 to class 201604, in which were 2,482 D boars, 201 D gilts, 1,154 L boars, 313 L gilts, 466 Y boars and 97 Y gilts. Genetic marker of SY11 on chromosome X located at 73cM was designated as the skeletal integrity gene. The variation of DNA fragment size of 9 fragments were 161~177bp with a 2bp difference in size. Analysis on allelic variants in various breed and gender by using a higher pig head sold in auction, there were 175+0 and 171+0 genotypes in D boars, 171+4 and 175+0 genotypes in D gilts, 171+0 and 167+0 genotypes in L boars, 167+4 and 171+0 genotypes in L gilts, 171+0 and 169+0 genotypes in Y boars, 171+0 and 169+2 genotypes in Y gilts. Major allelic variants of skeletal integrity gene regardless of gender were 175, 171 and 167bp in D, 171 and 167bp in L, and 171 and 169bp in Y, respectively. Conformation contest was carried out in each class to select objectively a champion of tall pig in each breed and each gender, hence, a total of 204 head of champion pig with a tall conformation was selected. The major allelic genotype for those of tall champion pigs were shown as 175+0, 171+0 and 171+0 in D, L and Y, respectively. Utilization of allelic variants of the skeletal integrity gene on sexual chromosome as gene barcodes with conformation data to select tall pig lines within breed, it could be beneficial to establish genomic databank on gene-linked economic trait for conformation selection of tall pig breeds.
Key Words: Breeding pig, Genetic marker, Conformation contest
  
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