Genetic diversity analysis of Fenghui Xiaying Red-Brand country chicken by microsatellite markers
D. Y. Lin(1), S. J. Tzeng(2), F. H. Yang(3), Y. Y. Lai(1), H. L. Lin(1) and M. C. Wu(1) (1)Livestock Research Institute(LRI), Council of Agriculture (2)Chung Hwa University of Medical Technology (3)Fenghui Breeder Farm
In order to evaluate genetic variation of Fenghui Xiaying Red-Brand country chicken flock. we use a set of 24 microsatellite markers recommended by FAO to analyze 48 candidate bred chickens from this flock. All the microsatellites were polymorphic. The average allelic number was 3.8, ranged from 2 to 8 per locus. The expected heterozygosity ranged from 0.118 to 0.822, and the average expected heterozygosity was 0.49±0.20. The observed heterozygosity of the population ranged from 0 to 0.688, and the average observed heterozygosity was 0.349±0.195. The estimated average polymorphic information content (PIC) was 0.434±0.194. In 24 markers, six markers were highly informative with polymorphism information content (PIC ≥ 0.50), eleven markers were reasonably informative(0.5 > PIC ≥ 0.25) and the other seven markers were slightly informative (PIC < 0.25). These results could be provided basic molecular information for the research on the germplasm characteristics of the population.