第五十一卷(2022)
94. 即時聚合酶鏈鎖反應檢測豬UBE3C基因點突變多態性之基因型分析
朱家德(1) 林德育(1) 賴永裕(1) 吳明哲(1) 張秀鑾(2)
(1)行政院農業委員會畜產試驗所 (2)國立屏東科技大學
肌間脂肪含量（IMF，%）為影響肌肉柔嫩度、多汁性、氣味及風味關鍵因子之一。研究指出，杜洛克豬隻泛素蛋白連接酶（ubiquitin protein ligase E3C, UBE3C）AA基因型者背最長肌之IMF （%） 顯著較AG型者為高（5.963 ± 0.339 vs. 4.169 ± 0.555%, P < 0.05）。本試驗旨在應用螢光引子（FAM/HEX-labelled Primer）標記之即時聚合酶連鎖反應（Real-time PCR）技術，發展我國商業豬種UBR3C-intron6突變點多態性檢測法。逢機選取72頭杜洛克、44頭藍瑞斯、17頭約克夏與47頭黑豬，進行g.1586399A>G基因型鑑定與IMF （%） 測定。初步結果顯示，在檢測之三個國際商業豬種族群，均無多態性。公系豬杜洛克種以野生型A交替基因為主 （基因頻率0.95），而母系藍瑞斯與約克夏豬種，則均為突變型G交替基因。然黑豬樣本中，野生型A交替基因頻率為0.65。UBE3C基因該突變點基因型與黑豬背最長肌IMF含量之關係，仍待進一步釐清。
關鍵語：豬、泛素蛋白連接酶、即時聚合酶鏈鎖反應
Genotypic analysis of single nucleotide polymorphism on UBE3C gene in commercial pigs by Real-time PCR platform
C. T. Chu(1), D. Y. Lin(1), Y. Y. Lai(1), M. C. Wu(1) and H. L. Chang(2)
(1)Taiwan Livestock Research Institute
(2)National Pingtung University of Science and Technology
Intramuscular fat content (IMF, %) is one of the key factors relating to pork tenderness, juiciness, odor, and flavor. Research reported that significantly higher IMF (%) of the longissimus dorsi muscle (LD) was found in Duroc pigs with AA genotype than those of AG type (5.963 ± 0.339 vs. 4.169 ± 0.555%, P < 0.05). The aim of this study was to develop a genotyping method for SNP of UBR3C-intron6 in commercial pig breeds using real-time PCR technology labeled with fluorescent primers (FAM/HEX-labelled Primer). The 72 Duroc, 44 Landrace, 17 Yorkshire, and 47 black pigs were randomly sampled for g.1586399A>G genotyping and IMF (%) measured. Preliminary results showed no polymorphisms in the three international commercial breeds were observed. The paternal breed, Duroc, was dominated by the wild-type A allele with a frequency of 0.95, while no wild-type allele was found in samples from the maternal breeds, Landrace, and Yorkshire. However, in the black pig samples, the frequency of the wild-type allele A was 0.65. The relationship between the point mutation genotype of UBE3C gene and the IMF content of LD in black pigs remains to be further elucidated.
Key Words: Pig, UBE3C gene, Real-time PCR
  
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