第三十三卷(2004)
二細胞期與八細胞期高繁殖力豬胚外科採集時間之探討
鄭連春(1) 劉振發(1) 林傳宗(1) 顏念慈(1) 陳立人(1) 張秀鑾(2) 吳明哲(1)
(1)行政院農業委員會畜產試驗所 (2)國立屏東科技大學
本試驗旨在探討獲得二細胞期與八細胞期豬胚之適當外科採集時間。應用七月齡以上之藍瑞斯女豬經律期媒（Regumate）連續餵飼 15 天後，隔天 2000 IU 孕馬血清激性腺素（Pregnant mare serum gonadotropin, PMSG）之肌肉注射，間隔 72∼78 小時再注射1500 IU 人類絨毛膜激性腺素（Human chronic gonadotropin, HCG），所有誘發發情之女豬於 HCG 注射後 24 及 30 小時，以人工授精方法進行複次配種，分別在第一次配種後 48∼50 小時、66∼68 小時、78∼80 小時、89∼91 小時、95∼97 小時進行外科手術取胚，以尋找獲得二細胞期與八細胞期豬胚之適當時間。結果所得之二細胞期與八細胞期豬胚比率分別在 48∼50 小時者(n=12)為49.3及 0%、在66∼68 小時者(n=3)為 10.4 及1.3%、在78∼80 小時者(n=4)為1.4及14.8%、在89∼91 小時者(n=3)為3.7及 9.3%、在95∼97小時者(n=3)為0 及22.6%。此結果顯示二細胞期豬胚外科採集時間在第一次配種後 48∼50 小時較佳，而八細胞期豬胚外科採集時間在第一次配種後 95∼97小時較佳。
關鍵語：外科採集、二細胞期豬胚、八細胞期豬胚
STUDIES ON SURGICAL COLLECTION DURATION OF 2-CELL
AND 8-CELL STAGE OF PORCINE EMBRYOS
L. C. Cheng(1), J. F. Liou(1), C. T. Lin(1), N. T. Yen(1), L.R. Chen(1), H. L. Chang(2), and M. C. Wu(1)
(1)Livestock Research Institute, Council of Agriculture, Executive Yuan
(2)National Pingtung University of Science and Technology
The purposes of this study were to find suitable surgical collection times to flush more 2-cell and 8-cell stage of pig embryos. Gilts at seven months of age or older were synchronized by feeding with 20 mg of Regumate daily for fifteen days, then received 1500 IU PMSG and 1000 IU HCG on 24 hrs and 72 to 78 hrs after synchronized, and artificially inseminated at 24 h and 30 h after HCG was injected. Five different surgical times (48 to 50 h, 66 to 68 h, 78 to 80 h, 89 to 91 h and 95 to 97 h after the first artificially inseminated) were compared to find the optimum time for recovering more 2-cell or 8-cell stage embryos. The frequency of 2-cell and 8-cell stage of five different surgical times were 49.3 and 0% at 48 to 50 h (n=12), 10.4 and 1.3% at 66 to 68 h (n=3), 1.4 and 14.8% at 78 to 80 h (n=4), 3.7 and 9.3% at 89 to 91 h (n=3) and 0 and 22.6% at 95 to 97h (n=3), respectively. The results showed that 48 to 50 h after the first artificially inseminated for recovering more 2-cell stage embryos was better surgical time, and 95 to 97 h after the first artificially inseminated for more 8-cell stage embryos was the better surgical duration.
Key Words: Surgical collection, 2-cell stage of pig embryo, 8-cell stage embryo.
  
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