第五十二卷(2023) agrkb - 2023-12-27 42. 即時聚合酶鏈鎖反應檢測商用土雞與蛋雞 OC-116 基因點突變之基因型分析 朱家德(1) 林德育(1) 賴永裕(1) 吳明哲(1) 張秀鑾(2) (1)農業部畜產試驗所 (2)國立屏東科技大學 種蛋孵化率是影響市場雛雞供給量與經濟效益的關鍵因素之一，而種蛋孵化率高低顯著受 蛋殼厚薄程度所影響。本試驗告在應用即時聚合酶鏈鎖反應之競爭性交替基因特異性聚合酶鏈鎖反應 （kompetitive allele-specific polymerase chain reaction, KASP）技術，發展我國影 響蛋殼厚度候選基因 （ovocleidin-116, OC-116） 之突變點多態性檢測法。隨機抽取商業紅 羽土雞、烏骨雞與龍門蛋雞各 50 隻進行 DNA 萃取，檢測 OC-116-C1336G 基因型頻率分布。初步結果顯示商業紅羽土雞、烏骨雞及龍門蛋雞的 C （cytosine） 交替基因頻率分別 為 0.85、0.2 及 1。未來，會持續收集商業紅羽土雞不同週齡種蛋孵化記錄，並進行 OC-116- C1336G 基因型與種蛋孵化率之關聯性分析，以釐清此基因型對性能表現的影響程度。 關鍵語：蛋殼厚度、孵化率、ovocleidin-116 基因、即時聚合酶鏈鎖反應 Genotypic analysis of single nucleotide polymorphism on OC-116 gene in commercial native chicken and layer by real-time PCR platform C. T. Chu(1), D. Y. Lin(1), Y. Y. Lai(1), M. C. Wu(1) and H. L. Chang(2) (1)Taiwan Livestock Research Institute (2)National Pingtung University of Science and Technology The hatchability is one of the key factors affecting the supply of market chicks and economic benefits, and it is significantly influenced by the eggshell thickness. The aim of this study was to develop a genotyping method for SNP of ovocleidin-116 in commercial native chicken and layer by using real-time PCR technology labeled with kompetitive allele-specific polymerase chain reaction (KASP). Commercial red feather native chicken, silkie, and Lohmann layers were randomly sampled for DNA extraction and genotyping with 50 birds per breed. Preliminary results indicated that the allelic frequency of cytosine in commercial red feather native chicken, silkie, and Lohmann layers were 0.85, 0.2, and 1, respectively. The effect of OC-116-C1336G on eggshell thickness and hatchability will be further investigated to confirm the essential influence. Key Words: Eggshell thickness, Hatchability, Ovocleidin-116 gene, Real-time PCR 44. 豬隻產仔性狀候選標記 EXOC4 頻率分析 44. 豬隻產仔性狀候選標記 EXOC4 頻率分析 廖仁寶(1) 王思雅(1) 盧昱諠(2) 陳若菁(1) 蔡秀容(1) 賴永裕(1) 劉桂柱(2) 程梅萍(1) (1)農業部畜產試驗所 (2)台灣區種豬產業協會 EXOC4 遺傳標記為豬隻產仔數性狀候選標記之一，本研究目的為篩檢種豬場種豬之 EXOC4 基因型，以暸解文獻所指出之有利基因型或對偶基因的頻率，進一步評估標記之產 業適用性。自 9 家種豬場選取 942 頭豬隻 DNA 樣品，包括杜洛克 371 個、藍瑞斯 414 個 及約克夏 157 個。分析結果顯示，所有檢測豬隻 CC、CT 及 TT 基因型的分布百分比，分 別為 66.35、28.13 及 5.52%﹔個別品種杜洛克、藍瑞斯及約克夏豬的 CC、CT 及 TT 基因型 百分比，則分別為 50.67、38.54、10.78% vs. 70.53、27.05、2.42% vs. 92.36、6.37、1.27%。 分析 109 頭具產仔數性狀的母豬基因型，CC、CT 及 TT 型百分比分別為 77.06、18.35 及 4.59%，另由基因型與產仔數性狀之相關性初步分析顯示，CT 型之杜洛克與藍瑞斯母豬比 CC 與 TT 型者有較高的平均產仔數。惟此部分尚須持續收集產仔性狀資料、分析及驗證， 俾便評估 EXOC4 納入種豬選育標記的適用性。 關鍵語：候選標記、產仔數性狀、種豬 Frequency analysis of a litter size trait candidate marker EXOC4 in stock pigs R. B. Liaw(1), S. Y. Wang(1), Y. S. Lu(2), J. C. Chen(1), H. J. Tsai(1), Y. Y. Lai(1), K. J. Liu(2) and M. P. Cheng(1) (1)Taiwan Livestock Research Institute, Ministry of Agriculture (2)Formosan Farmers Association for Swine Improvement The EXOC4 genetic marker is one of the candidate markers for pig litter size traits. The purpose of this study was to screen the EXOC4 genotypes of stock pigs in breeding farms to understand the frequency of favorable genotypes or alleles indicated in the literature and to further evaluate the markers for industrial applicability. DNA samples were collected from 942 pigs of 9 breeding farms, including 371 Duroc, 414 Landrace, and 157 Yorkshire. The analysis results show that the percentages of CC, CT and TT genotypes in all tested pigs were 66.35, 28.13 and 5.52%, respectively﹔ the CC, CT, and TT genotype percentages of individual breeds of Duroc, Landrace, and Yorkshire pigs are 50.67, 38.54, 10.78% vs. 70.53, 27.05, 2.42% vs. 92.36, 6.37, 1.27%, respectively. Analyzing the genotypes of 109 sows with litter size traits, the percentages of CC, CT, and TT genotypes were 77.06, 18.35, and 4.59% respectively. In addition, preliminary analysis of the association between genotypes and litter size traits shows that the Duroc and Landrace sows with CT genotype had higher mean litter size than CC and TT ones. However, this part still needs to continue to collect, analyze, and verify data on litter size traits in order to evaluate the applicability of EXOC4 as a breeding marker for pigs. Key Words: Candidate marker, Litter size trait, Stock pig 57. 台灣土雞近親品系台畜一號種公雞與其後代體重整齊度分析 57. 台灣土雞近親品系台畜一號種公雞與其後代體重整齊度分析 林德育(1) 曾淑貞(2) 賴永裕(1) 洪哲明(1) 蔡銘洋(1) 朱家德(1) (1)農業部畜產試驗所 (2)中華醫事科技大學 體重整齊度是一個非常重要的雞隻管理工具，亦為雞隻的重要經濟性狀。整齊度不佳確實 會影響雞群生產性能與動物福利，而變異係數是評估整齊度的適當指標。為探討種公雞體 重對其後代上市體重整齊度的影響，本研究以台灣土雞近親品系台畜一號四個近親品系 (L7、L9、L11 及 L12)G14 世代 266 隻種雞與 G15 世代 2,017 隻生長雞之 16 週齡體重進 行整齊度分析。將各品象種公雞與種母雞 16 週齡體重大小分成高體重組、中體重組及低體 重組。分析中體重組與全部種公雞組的後代之 16 週齡體重，並比較其變異係數。結果顯示 雞隻 16 週齡體重之變異係數在種公雞比較組間存在顯著性差異(P < 0.05)，中體重組種公 雞較全部種公雞組種公雞的後代有較低的變異係數，反映出有較佳的整齊度。本研究所得 的結果可作為種雞業者生產肉用雛雞選用種公雞的參考。 關鍵語：雞、體重、整齊度 Analysis on the uniformity of body weight of breeding roosters and their offsprings in Taiwan native chicken Taizhu No. 1 inbred lines D. Y. Lin(1), S. J. Tzeng(2), Y. Y. Lai(1), C. M. Hung(1), M. Y. Tsai(1) and C. T. Chu(1) (1)Livestock Research Institute, Ministry of Agriculture (2)Chung Hwa University of Medical Technology Uniformity of body weight is a very important management tool and an important economic trait in chicken industry. In order to explore the impact of the body weight of breeding roosters on the uniformity of the marketing body weight of their offspring, this study used the 16-week-old body weights of 266 breeder chickens of the G14 generation and 2,017 growing chickens of the G15 generation of four inbred lines of Taiwan native chicken Taizhu No. 1 to conduct uniformity analysis. The 16-week-old body weight of male and female chickens of each line was divided into high- weight group, medium-weight group (M) and low-weight group. We analyze the 16-week-old body weight of the offspring between the medium body weight group and all breeding roosters group (AL), and compare their coefficients of variation. The results showed that there were significant differences in the coefficient of variation of body weight at 16 weeks of age between the comparison groups of breeding roosters (P < 0.05). The offspring of breeding roosters in group M have a lower coefficient of variation than the offspring of roosters in group AL, reflecting better uniformity. The results obtained in this study can be used as a reference for chicken breeders to select breeding roosters for producing broiler chicks. Key Words: Chicken, Body weight, Uniformity 98. 豬 GPAT1 與 PRKAG 3 基因點突變多態性之基因型分析 98. 豬 GPAT1 與 PRKAG 3 基因點突變多態性之基因型分析 朱家德(l) 林德育(l) 賴永裕(l) 吳明哲(l) 張秀鑾(2) (l)農業部畜產試驗所 (2)國立屏東科技大學 肌內脂肪(IMF）含量為影響肌肉柔嫩度、多汁性、氣味及風味關鍵因子之一。文獻指出， GPAT1 (Glycerol-3-phosphate acyltransferase l） 與 PRKAG3 (protein kinase AMP-activated non-catalytic subunit gamma 3） 基因點突變顯著影響背最長肌 IMF 與 pH 值。本研究旨在 應用即時聚合酶鏈鎖反應技術，發展影響豬隻 IMF 含量之候選基因突變點多態性檢測法。 逢機選取 13 頭杜洛克、13 頭藍瑞斯、8 頭約克夏與 13 頭黑豬進行多態性位點基因型鑑定， 初步結果顯示：前述品種豬隻 GPATl-SfaNI 位點 CC 基因型頻率依序為 3l、l00、l00 與 62%﹔GPATl-BbvI 位點 CC 基因型則依序為 39、39、63 與 l00%。PRKAG3-A325G 位點 AA 基因型頻率分別為 46、23.0、75 與 23%。未來將近一步分析 GPATl 與 PRKAG3 基因 前述突變點與豬隻背最長肌 IMF 含量相聯性，釐清其影響程度。 關鍵語：豬、GPATl 基因、PRKAG3 基因、即時聚合酶鏈鎖反應 Genotypic analysis of single nucleotide polymorphism on GPATl and PRKAG3 genes in commercial pigs C. T. Chu(l), D. Y. Lin(l), Y. Y. Lai(l), M. C. Wu(l) and H. L. Chang(2) (l)Taiwan Livestock Research Institute (2)National Pingtung University of Science and Technology Intramuscular fat (IMF) content is one of the key factors affecting pork tenderness, juiciness, odor, and flavor. The literature indicated that GPATl (Glycerol-3-phosphate acyltransferase l) and PRKAG3 (protein kinase AMP-activated non-catalytic subunit gamma 3) genes significantly affect the IMF content and pH value of the longissimus dorsi muscle in pig. This study aims to develop a genotyping method in pig breeds using real-time PCR technology. Thirteen Duroc, thirteen Landrace, eight Yorkshire, and thirteen black pigs were randomly sampled for genotyping. Preliminary results showed that the CC genotype frequencies of the above breeds at the GPATl- SfaNI site were 3l, l00, l00, and 62%, respectively﹔ the GPATl-BbvI site were 39, 39, 63 and l00%, respectively. However, the AA genotype frequencies for PRKAG3-A325G were 46, 23, 75, and 23%, respectively. In the future, we will further analyze the correlation between the above GPATl and PRKAG3 gene mutations and the IMF content of pig longissimus dorsi muscle to clarify their impact. Key Words: Pig, GPATl gene, PRKAG3 gene, Real-time PCR 105. 季節對杜洛克公豬精液品質之影響 105. 季節對杜洛克公豬精液品質之影響 彭麟量(1) 郭廷雍(1) 林秀蓮(1) 賴永裕(1) 林德育(1) 許順貴(2) 劉桂柱(2) 楊鎮榮(1) (1)農業部畜產試驗所 (2)台灣區種豬產業協會 氣候變遷走全球面臨的議題，環境溫度提高使熱帶及亞熱帶地區動物飼養挑戰加劇，熱緊 迫會對公豬生殖力造成負面影響。試驗收集 2014 至 2015 年台灣區種豬協會拍賣之杜洛克 公豬精液，依採精月份分為 3-5 月（春季）、6-8 月（夏季）、9-11 月（秋季）及 12-2 月（冬 季），評估採精季節對精液量、活力、濃度、總精子數、存活力、頭帽與精子膜完整性、粒 線體去極化及精子染色質結構完整性之影響，結果顯示，杜洛克公豬精液濃度春季及冬季 顯著高於夏季及秋季（P < 0.05），總精子數春季及冬季顯著高於秋季（P < 0.05）﹔粒線體去 極化比例秋季顯著高於春季（P < 0.05），精子染色質結構完整性秋季顯著低於春季（P < 0.05），秋季收集之精液品質較春季差，綜上，杜洛克公豬的精液品質受季節影響，為因應 氣候變遷，未來選育公豬耐熱性能可考慮將熱季精液評估納入指標。 關鍵語：精液品質、流式細胞儀 Seasonal effect to semen quality of duroc boars L. L. Peng(1), T. Y. Kou(1), H. L. Lin(1), Y. Y. Lai(1), D. Y. Lin(1), S. K. Hsu(2), K. J. Liu (2) and J. R. Yang(1) (1)Taiwan Livestock Research Institute, Ministry of Agriculture. (2)Formosan Farmers Association for Swine Improvement Climate change is a global issue. Increased ambient temperatures will intensify the challenges of raising animal in tropical and subtropical areas. Heat stress have a negative impact on boar fertility. This experiment collected the semen of Duroc boars auctioned by the Formosan Farmers Association for Swine Improvement from 2014 to 2015. Samples were divided into 4 group by March-May (spring), June-August (summer), September-November (autumn) and December- February (winter) and evaluated the effect of season on semen quality traits such as volume, motility, concentration, total sperm number, viability, acrosome and sperm membrane integrity, mitochondria depolarization and sperm chromosome structural integrity. The results showed that the semen concentration of Duroc boars in spring and winter were significantly higher than that in summer and autumn (P < 0.05). The total sperm number in spring and winter were significantly higher than in autumn (P < 0.05). The proportion of sperm mitochondria depolarization in autumn was higher than in spring (P < 0.05). The sperm chromatin structure integrity in autumn was lower than in spring (P < 0.05). It shows that the semen quality in autumn was worse than in spring. The semen quality of Duroc boars are affected by season. In order to cope with climate change, semen evaluation indicators in hot seasons can be considered selection of boars for heat tolerance in the future. Key Words: Semen quality, Flow cytometry 112. 臺灣登錄山羊微衛星遺傳標記基因多樣性分析一努比亞山羊族群析 112. 臺灣登錄山羊微衛星遺傳標記基因多樣性分析一努比亞山羊族群 謝佳容(1) 陳若菁(1) 林德育(1) (1)農業部畜產試驗所遺傳生理組 本研究藉由微衛星遺傳標記了解目前臺灣登錄山羊族群的基因多樣性，以國際農糧組織建 置之山羊微衛星標記檢測平台中 MAF065、MCM527、TCRVB6、SRCRSP9、OarFCB48、 CSRD247、SRCRSP23、INRA063、OarAE54 與 DRBP1 等 10 個微衛星標記分析南部某種 羊場 135 頭努比亞山羊。其檢測結果顯示共有 152 個交替基因，平均每個基因座其有 7.6 個 交替基因。並且其期望異質度介於 0.546 至 0.776，平均為 0.668，觀測異質度則介於 0.541 至 0.807，平均為 0.673，多態性訊息含量介於 0.492 至 0.744，其平均為 0.624；在此 10 組 微衛星標記中，有 9 組具有高多態性訊息含量（PIC>0.5）。本研究結果顯示此 9 組微衛星 標記能有效偵測努比亞山羊族群之遺傳多樣性，可以此進一步了解其遺傳結構與親緣關係， 作為未來品種選育之參考。 關鍵語：努比亞山羊、遺傳多樣性、微衛星標記 The genetic diversity analysis with microsatellite markers of Taiwan registered goats-Nubian goat population C. J. Hsieh(1), J. C. Chen(1) and D. Y. Lin(1) (1)Genetics and Physiology Division, Taiwan Livestock Research Institute, Ministry of Agriculture This study was conducted to elucidate the genetic diversity of registered goats in Taiwan and focused on the Nubian goat population from a breeding goat farm in southern Taiwan. A total of 135 heads of Nubian goats were involved in this study. Ten FAO-approved microsatellite markers, including MAF065, MCM527, TCRVB6, SRCRSP9, OarFCB48, CSRD247, SRCRSP23, INRA063, OarAE54, and DRBP1 were applied to analyze the genetic diversity of Nubian goats. The results showed that the expected heterozygosity values were 0.546 to 0.776 and had a mean value of 0.668. The observed heterozygosity values ranged from 0.541 to 0.807 and had a mean value of 0.673. The polymorphic information content (PIC) was estimated with a mean of 0.624 and ranged from 0.492 to 0.744. Nine of 10 microsatellite markers indicated highly informative (PIC > 0.5). The results showed that based on these nine microsatellite markers, the genetic diversity of black goat populations could be monitored, and the genetic structure and phylogenetics would be analyzed in the future. Key Words: Nubian goats, Genetic diversity, Microsatellite markers 123. 不同超音波導引探頭應用於淘汰牛隻體內撿卵之效果比較 123. 不同超音波導引探頭應用於淘汰牛隻體內撿卵之效果比較 郭廷雍(1) 曲鳳翔(1) 林信宏(2) 李佳馨(3) 李佳蓉(4) 梁世祥(4) 范耕榛(4) 楊鎮榮(1) (1)農業部畜產試驗所 遺傳生理組 (2)農業部畜產試驗所 南區分所 (3)農業部畜產試驗所北區分所 (4)農業部畜產試驗所 畜產經營組 乳牛產業可以利用體內取卵法搭配體外胚生產法（OPU-IVEP）產製可移置胚，本法對於最 優良的牛群而言其備短短幾年內能迅速擴增及改良後代牛群最快速的優勢，即加速其高遺 傳優勢的潛力母牛和優異公牛中獲得後代的能力以實現更快的遺傳進展，並且解決了可移 置胚數量受限的瓶頸並讓遺傳優勢等級較高的胚胎的移植比例更高。OPU 系統由 3 個主 要組件組成，帶有適當探頭的超音波掃描儀、抽吸幫浦和連接到卵母細胞收集管的針引導 系統，讓牛隻體內取卵得以實現，本研究之目的為比較法式及日式超音波探頭及其針頭形 式應用於淘汰牛隻體內撿卵之效果，結果顯示卵子回收率在法式及日式探頭分別為 45%及 54%，回收卵子之卵丘細胞完整率為 75%及 85%及每頭牛隻平均約收集 3.5 顆卵子，綜上 所述，有鑑於其備卵丘細胞完整性評估對卵母細胞（COC）成熟和未來發育能力的重要性， 必須針對給定象統評估抽吸程序對 COC 造成的任何損害，以便採取預防措施以提高相關 卵子回收率及減少對卵丘細胞完整性的破壞。 關鍵語：體內取卵、牛、卵子、回收率 Comparison of the effects using different ultrasonic guidance probes on oocyte collection in culled cows T. Y. Kuo(1), F. H. Chu(1), H. H. Lin(2), C. X. Lee(3), C. R. Lee(4), S. S. Liang(4), G. J. Fan(4) and C. J. Yang(1) (1)Genetic and Physiology Division, Livestock Research Institute, M.A. (2)South region branch, Livestock Research Institute, M.A (3)Nouth region branch, Livestock Research Institute, M.A (4)Livestock Management Division, Livestock Research Institute, M.A. An OPU system consists of 3 ma吋or components: an ultrasonographic scanner with an appropriate transducer (probe), an aspiration pump, and a needle guidance system connected to an oocyte collecting tube. The aim of this study was the comparison of the effects using different ultrasonic guidance probes and needles on oocyte collection in culled cows. The result shows that oocytes recovery rate guiding by France and Japanese probe were 45% and 54%. Rate of recovery oocytes with intact cumulus cell were 75%及 85%. Averagely, about 3.5 of oocytes were collected on each culled cow. In summary, given the importance of an intact cumulus cell investment for oocyte maturation and future developmental capacity, any damage to the COC caused by the aspiration procedure has to be assessed for a given system so that preventive procedures can be taken. Key Words: Ovum picks up, Bovine, Oocyte, Recovery rate 135. 番鴨 iPSC 細胞株建立與水禽小病毒感染測試 135. 番鴨 iPSC 細胞株建立與水禽小病毒感染測試 劉振發(1) 張家語(1) 曲鳳翔(1) 陳立人(1) (1)農業部畜產試驗所 研究目的為建立番鴨誘導多能性幹細胞（mdiPSC），並進行水禽小病毒感染測試，評估此細 胞株作為疫苗生產之潛力。試驗利用慢病毒將 LIN28, NANOG, SOX2, OCT3/4, KLF4 和 C-MYC 等轉錄因子轉染番鴨胚胎纖維母細胞（mdEFs），經約 7-10 天後 mdEFs 逐漸由 梭狀轉為圓形之上皮細胞形態，持續培養 35-40 天後則形成類似幹細胞群落形態。 mdiPSC 體外培養已超過 45 繼代（380 天），經分化多能性細胞標誌 AP 與 PAS 染色後 呈現陽性反應﹔使用懸浮小滴培養技術培養，其有形成類胚體能力﹔顯示所建立之番鴨誘 導多能性幹細胞其有分化多能性與分化潛能。以聚合晦連鎖反應進行檢測，證實經水禽小 病毒直接感染後之 mdiPSCs，以及受到經成染 mdiPSCs 之回收細胞液共培養的 mdiPSCs， 結果均呈陽性反應，證實水禽小病毒可感染本研究所建立的 mdiPSC，並可以在其細胞內 順利複製病毒顆粒，顯示本研究所建立的 mdiPSC 可作為水禽小病毒宿主細胞及其疫苗生 產的潛力。 關鍵語：番鴨、胚胎纖維母細胞、誘導多能性幹細胞、水禽小病毒 Establishment of induced pluripotent stem cell line in muscovy duck and its waterfowl parvovirus infection test J. F. Liou(1), C. Y. Chang(1), F. H. Chu(1) and L. R. Chen(1) (1)Taiwan Livestock Research Institute, Ministry of Agriculture. The purpose of this study was to establish muscovy duck induced pluripotent stem cell (mdiPSC) lines and to evaluate their potential for vaccine production. The results showed that the morphology of muscovy duck embryonic fibroblasts (mdEFs) transformed into colony type from spindle type 7- 10 days after being infected with lentivirus, which was constructed with reprogramming transcription factors of LIN28, NANOG, SOX2, OCT3/4, KLF4 and C-MYC. The transformed cells have been maintained in vitro for more than 45 passages (380 days). These putative mdiPSCs continuously expressed pluripotent markers of avian stem cells including AP and PAS antigens. The capability of embryoid body formation of the putative mdiPSCs was excellent when induced by handing-drop culture. These results demonstrated that the mdiPSC line established in this study were pluripotent. The specific 539 bp DNA fragment was recovered in the mdiPSCs after infection with waterfowl parvovirus, and also the mdiPSCs infected with the cellular content recovered from previously waterfowl parvovirus-infected mdiPSCs when detected by polymerase chain reaction. This results shown that mdiPSC cell line with the potential for production of the vaccines against waterfowl parvovirus. Key Words: Muscovy duck, Embryonic fibroblasts, IPSC, Waterfowl parvovirus 137. 畜產試驗所實驗兔線上銷售系統上線 137. 畜產試驗所實驗兔線上銷售系統上線 蔡佩均(1) 楊鎮榮(1) (1)農業部畜產試驗所遺傳生理組 本研究的目的是提高畜產試驗所在網路上兔隻生產銷售之品質和效率。如今網頁使用者體 驗越來越受到重視，尤其是手機使用者，因此開發使用者友善的線上銷售顯得格外重要。 這個研究優化並升級實驗兔銷售象統，建構客戶新增、使用者管理、線上訂購、訂單管理 及友善查詢象統，讓使用者及管理者共同監控訂單執行的現況，優化使用體驗。我們期望 藉此提升實驗兔的供應服務品質，增加銷售量。 關鍵語：實驗兔、銷售系統、網頁 The Online Sales System for Experimental Rabbits Launched in Taiwan Livestock Research Institute P. Tsai(1) and J. Yang(1) (1)Genetics and Physiology Division, Taiwan Livestock Research Institute, Ministry of Agriculture The purpose of this study is to improve the quality and efficiency of rabbit production and sales on the Internet by the Taiwan Livestock Research Institute. Nowadays, the experience of Internet user is paying more and more attention, especially for mobile phone users. Therefore, it is more important to develop user-friendly web pages. This study optimized and upgraded the experimental rabbit sales webpage. Create customer lists, user management, online ordering and friendly inquiry systems. Allow users and managers to jointly monitor order execution. We expect to increase sales through this optimized experimental rabbit supply system. Key Words: Experimental rabbit, Sales system, Webpage 143. 利用豬誘導多能性幹細胞建構組織工程血管 143. 利用豬誘導多能性幹細胞建構組織工程血管 廖御靜(1) 楊鎮榮(1) (1)農業部畜產試驗所遺傳生理組 發展利用多能性幹細胞（pluripotent stem cells）建構組織工程血管（tissue-engineered vascular grafts, TEVGs）之技術，對治療人類心血管疾病其有相當大之潛力。然而，目前利用豬誘導多能性幹細胞（porcine induced pluripotent stem cells, piPSCs）建構 TEVGs 之研究仍於啟蒙階段，技術尚待開發以利臨床前研究所需。本研究建立 piPSCs 分化為平滑肌細胞（smooth muscle cells, SMCs）之方法，並利用 3D 模具以自體組合方式將 SMCs 建構成其彈性之環狀構造，其再經多次堆疊後可建構成小尺寸之 TEVGs，此技術無需支架即可利用 piPSCs 分化之 SMCs 產製 TEVGs。未來有待強化其結構後近一步驗證移植於動物體內之表現，以實現其應用潛力。 關鍵語：豬、誘導多能性幹細胞、平滑肌細胞、組織工程血管 Generation of tissue-engineered vascular grafts using porcine induced pluripotent stem cells Y. J. Liao(1) and J. R. Yang(1) (1)Genetics and Physiology 0ivision, Taiwan Livestock Research Institute, Ministry of Agriculture 0evelopment of tissue-engineered vascular grafts (TEVGs) using pluripotent stem cells holds significant potential for treating human cardiovascular diseases. However, current research involving the use of porcine induced pluripotent stem cells (piPSCs) to construct TEVGs is still at the attempt stage, and the technology requires further development for preclinical studies. Here, we report the generation of smooth muscle cells (SMCs) derived from piPSCs and the utilization of a 30 mold to autonomously assemble SMCs into a flexible tubular structure, which can be used to create small-sized TEVGs through multiple layering. This technique can construct TEVGs without scaffolds. To fulfill their application potential, further validation through in vivo transplantation studies in animal models after strengthening the structure is required. Key Words: Pigs, Pluripotent stem cells, Smooth muscle cells, Tissue-engineered vascular grafts 144. 蘭嶼豬生物醫學影像之建構 144. 蘭嶼豬生物醫學影像之建構 楊鎮榮(1) 廖御靜(1) (1)農業部畜產試驗所遺傳生理組 蘭嶼豬（Lanyu pigs）為臺灣生醫用小型豬種，已廣泛應用於基礎研究、心血管疾病、組織工程與藥理學等研究，對暸解與治療人類疾病具有相當大之潛力。然而，對蘭嶼豬的解剖構造暸解有限，加上解剖圖鑑與生物醫學影像資料的缺乏，使動物試驗無法精準有效的操作。本研究建立蘭嶼豬公豬與母豬腦、腹部脂肪與生殖器官之磁振造影（magnetic resonance imaging, MRI）影像，以及全身之電腦斷層掃描（computed tomography, CT）影像，建立完整生物醫學影像資料庫。此影像為連續切面並可重構為 3D 結構，於電腦上可即時查閱，有利於動物試驗時精準有效的操作，並有效減少試驗動物使用量。未來此資料庫將線上開放使用，對試驗研究與教學使用將有相當大之助益。 關鍵語：蘭嶼豬、磁振造影、電腦斷層掃描 Establishment of biomedical imaging in Lanyu pigs J. R. Yang(1) and Y. J. Liao(1) (1)Genetics and Physiology 0ivision, Taiwan Livestock Research Institute, Ministry of Agriculture Lanyu pigs are a miniature swine breed used extensively in Taiwan for biomedical research, such as in the fields of basic research, cardiovascular diseases, tissue engineering, and pharmacology. They hold significant potential for understanding and treating human diseases. However, the understanding of the anatomical structures of Lanyu pigs is limited. Furthermore, the lack of anatomical atlases and biomedical imaging data hampers precise and effective procedures during animal research. Here, male and female Lanyu pigs are subjected to magnetic resonance imaging (MRI) of the brains, abdominal fat, and reproductive organs, and to computed tomography (CT) of whole bodies. This comprehensive biomedical imaging database consists of sequential cross- sections that can be reconstructed into 30 structures. The imaging allows for real-time review on a computer, promoting precise and efficient procedures during animal research and reducing the number of animals used. This database will be available online in the future, offering substantial benefits for experimental research and educational purposes. Key Words: Lanyu pigs, Magnetic resonance imaging, Computed tomography 153. 不同孵化時期提高溫度對雛雞品質及死亡率的影響 153. 不同孵化時期提高溫度對雛雞品質及死亡率的影響 郭曉芸(1) 張以恆(1) (1)農業部畜產試驗所 本試驗旨在探討於雞隻孵化前期與後期提高孵化溫度，對雛雞品質與死亡率的影響。試驗選用白肉雞受精蛋 300 枚，依蛋重平均分為 4 組，A 組為固定孵化期溫度 37.8C之對照組﹔ B 及 C 組分別於入孵後 4 至 7 天及 15 至 18 天，每提高 6 小時孵化溫度至 39.5C﹔D 組則合併 B、C 組處理。結果顯示，入孵 7 天後 B 及 D 組蛋失重比顯著高於對照組與 C 組，而 B、C 及 D 組孵化期均顯著低於對照組。各組總孵化率相似，而 C 組死亡集中於入孵晚期，D 組入孵中期死亡率顯著大於其他處理組，入孵晚期死亡率顯著小於其他組。雛雞體重於組間無顯著差異，然 D 組體溫顯著低於其他組別。綜上所述，不同孵化時期提高孵化溫度對雛雞體重無影響，然孵化前後期均進行提高溫度之處理，雛雞其有較低的體溫，可作為後續雞隻於溫度變化下體溫調節研究的之參考。 關鍵語：白肉雞、孵化溫度、雛雞品質、死亡率 Effects of increasing incubation temperature at different stages on chick quality and mortality H. Y. Kuo(1) and I. H. Chang(1) (1)Taiwan Livestock Research Institute, Ministry of Agriculture This experiment aimed to explore the effects of increasing incubation temperature in the early and late stages on chick quality and mortality. 300 fertilized broiler eggs were used and randomly divided into 4 groups according to egg weight. Group A had a fixed temperature of 37.8°C as a control﹔ Group B and C raised the incubation temperature to 39.5°C for 6 hours a day on days 4 to 7 and 15 to 18 after hatching, respectively﹔ Group D combined the treatments of Groups B and C. The results showed that the egg weight loss ratio of groups B and D was significantly higher than the control group and group C after 7 days of incubation. The incubation periods of groups B, C, and D were significantly shorter than the control group. The total hatchability of each group was similar﹔ however, the mortality in group C was higher in the late incubation period, while the mortality in group D during the middle incubation period was significantly higher and lower in the late period. There was no significant difference in chick body weight between groups, but the body temperature of group D was significantly lower than others. In summary, raising the incubation temperature at different incubation periods had no effect on the body weight of the chicks. However, if the incubation temperature was increased in both the early and late stages, the chicks would have a lower body temperature, which could be used as a reference for subsequent research on the regulation of chicken body temperature under temperature changes. Key Words: Broiler, Incubation temperature, Chick quality, Mortality 155. 乳牛活體採卵效能提升 155. 乳牛活體採卵效能提升 曲鳳翔(1) 郭廷雍(1) 陳裕信(2) 李佳蓉(2) (1)農業部畜產試驗所遺傳生理組 (2)農業部畜產試驗所畜產經營組 人工生殖技術應用旨在提高家畜經濟效益，在這些技術應用中，體外胚胎生產（IVEP）受到關注，其在畜牧產業中的應用不斷增加。全球牛胚移置數量近十年間成長 10 倍（20 萬∼ 200 萬／每年）其中以活體採卵配合胚體外生產（OPU-IVP）占大多數（2022 年已達 68% 以上），已成為改善牛隻繁殖育種效能及提升單位產量的重點研發方向。本研究的目的是在評估乳牛活體採卵（Ovum Pick Up）採集卵母細胞之效能，以直腸觸診方式固定卵巢利用超音波顯影探頭配合採卵探針，吸取卵巢濾泡中的卵子，這樣的操作過程需要嫻熟的技巧才能達到 60%以上的回收率。經過表面鍍層的取卵探針，能有效提升在超音波顯示螢幕上的顯影效果，相較傳統探針卵子平均可提升回收率 7%。 關鍵語：乳牛、活體採卵、胚體外生產 Improved Efficiency of Oocytes Collection from Dairy Cattle F. H. Chu(1), T. Y. Guo(1), Y. S. Chen(2) and J. R. Li(2) (1)Genetics and Physiology Division, Taiwan Livestock Research Institute, Ministry of Agriculture (2)Livestock Management Division, Taiwan Livestock Research Institute, Ministry of Agriculture The application of artificial reproductive technologies aims to improve the economic benefits of livestock. Among these technological applications, in vitro embryo production (IVEP) has attracted attention, and its application in the livestock industry continues to increase. The number of bovine embryo transfers worldwide has increased tenfold in the past decade (200,000 to 2 million / year), of which live egg collection combined with in vitro embryo production (OPU-IVP) accounts for the majority (reaching more than 68% in 2022), which has become The key research and development direction is to improve the efficiency of cattle breeding and increase unit output. The purpose of this study is to evaluate the effectiveness of Ovum Pick Up in collecting oocytes from dairy cows. The ovaries are fixed in the rectal veterinary examination method and the eggs in the ovarian follicles are aspirated using an ultrasonic imaging probe combined with an egg collection probe. In this way the operation process requires skilled skills to achieve a recovery rate of more than 60%. The surface- coated egg retrieval probe can effectively improve the development effect on the ultrasonic display screen, and can increase the recovery rate by an average of 7% compared with traditional probes. Key Words: Dairy cattle, Ovum Pick Up, Embryo in vitro production 162. 紅馬鹿鹿茸不同部位之生長因子含量分析測定 162. 紅馬鹿鹿茸不同部位之生長因子含量分析測定 郭廷雍(1)(2) 戴偉城(2) 林信宏(3) 曾進輝(3) 鄭炳霖(4) 林燦揚(4) (1)農業部畜產試驗所遺傳育種組 (2)國立成功大學生物科技與產業科學系 (3)農業部畜產試驗所南區分所 (4)苗票縣養鹿促進發展協會 近年研究報告顯示了鹿茸萃取物在醫學中的驚人應用，可開發為傷口再生癒合的臨床藥物。台灣紅馬鹿為外來品種紅鹿及馬鹿經雜交培育並長期飼養後已充分適應本地氣候之特殊高大型鹿品系，其鹿茸中的生長因子如胰島素生長因子（IGF）、成纖維細胞生長因子（FGF）及乙型轉化生長因子（TGF-β）等含量之相關研究仍待建立，本研究目的在分析台灣紅馬鹿茸內之各類生長因子含量。台灣紅馬鹿茸採茸後分為三分切段，自頂端至基部分別為頂部、中部及根部，以酵素結合免疫吸附分析法（ELISA）分析新鮮鹿茸中相關生長因子種類及含量。初步結果顯示新鮮紅馬鹿茸之總可溶性蛋白質含量為2.37mg/每克茸並以茸頂部位最高，頂部在IGF含量為最高分別為2909.5 pg/g及 FGF 為 0.6293 pg/mL，根部則以 TGF-β 含量 2243.2 pg/g 為最高。綜上所述，生長因子 IGF、FGF 及 TGF-β 其含量在各切段部位存在差異，本試驗可進一步供作相關鹿茸品質優劣鑑別之應用。 關鍵語：台灣紅馬鹿茸、夷島素生長因子、成纖維細胞生長因子、乙型轉化生長因子 The growth factor content of velvet of Taiwan red deer-like (Cervus elaphus) strain T. Y. Kuo(1)(2), W. T. Dai(2), H. H. Lin(3), C. H. Tseng(3), B. L. Cheng(4) and C. Y. Lin(4) (1)Genetic and physiology Division, Livestock Research Institute, M.A. (2)Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University. (3)South region branch, Livestock Research Institute, M.A (4)Association for Deer Raising and Development of Miaoli County Recent research reports have shown the amazing application of deer antler extract in pharmacological industry. The aim of this study was to investigate the growth factor content of Taiwan red deer-like. We used Enzyme-linked immunosorbent assay (ELISA) to analyze related growth factors content of velvets. Results showed that the tip of velvets had highest total protein content, which were 2.37 mg/g. The upper parts of fresh velvets had highest IGF and FGF content, which were 2909.5 pg/g and 0.6293 pg/mL. On the bottom part of velvets had highest TGF-β content, which was 2243.2 pg/g. In summary, there exist content differences of growth factor at each section. This study can be used for the identification of the quality of deer antler velvets. Key Words: Taiwan red deer-like, IGF, EGF, TGF-β 163. 應用精子新穎參數於種公豬精子耐凍性之評選 163. 應用精子新穎參數於種公豬精子耐凍性之評選 郭廷雍(1) 林信宏(2) 李耶瑩(3) 楊卓真(3) 楊國泰(4) 許順貴(5) (1)農業部畜產試驗所遺傳育種組 (2)農業部畜產試驗所南區分所 (3)國立嘉義大學動物科學系 (4)國立屏東科技大學動物科學與畜產系(5)屏東縣金山種豬場 研究顯示冷凍保存對公豬精子造成損害，應用新穎精子功能參數測定可用作篩選公豬個體是否其備精子耐凍性外亦可檢測出質量差的豬冷凍精液。因此本研究目的為應用新穎精子 功能參數於種公豬個體問精子耐凍性進行分析外並指出精子損傷的潛在指標，以評選繁殖 性能耐凍公豬、排除質差冷凍豬精及冷凍配方改善，試驗結果顯示不同公豬個體問冷凍傷害主要造成精子活動力及存活力的下降，在耐凍性不佳之個體公豬在精液解凍前後顯現精子活力下降 37.0% (P< 0.05)、前向運動性下降 22.1% (P< 0.05)、存活力下降 24.0% (P< 0.05)及精子頭帽完整性下降 8.3 % (P< 0.05)、精子 DNA 完整性下降 1.9%及氧化傷害增加 2%，顯示公豬間其精子耐凍能力存在個體差異。綜上所述，在進行精液冷凍保存前對耐凍性評估和精子耐凍評選將有利於使用冷凍解凍的公豬精液產品進行人工授精並可得到合理的生育成績。 關鍵語：公豬、精子、流式細胞儀、精子新穎參數 Application of novel sperm function in assessment of the freezability of boar ejaculates T. Y. Kuo(1), H. H. Lin(2), Y. Y. Lee(3), C. C. Yang(3), K. T. Yang(4) and S. K. Hsu(5) (1)) Genetic and physiology Division, Livestock Research Institute, M.A. (2)South region branch, Livestock Research Institute, M.A (3)Department of Animal Science, National Chiayi University (4)Department of Animal Science, National Pingtung University of Science & Technology (5)Jinshan Breeding farm, Ligang Township, Pingtung County, Taiwan Damage to boar sperm from cryopreservation is known to result in reduced motility, viability and litter size. Novel sperm function assays were used to detect poor quality frozen porcine semen prior to use and thus improve AI success. The purpose of this study was using novel sperm function assessment to characterize cryopreserved boar spermatozoa. Low freezability boar showed about 37.0% (P < 0.05) motility decrease, 22.1% (P < 0.05) progressive motility decrease, 24.0% (P < 0.05) sperm viability decrease and 8.3 % (P < 0.05) acrosome integrity decrease, 1.9% intact DNA decrease and 2% of oxidative stress increased after frozen-thawed were observed. In summary, freezable ejaculates assessment and sperm selection before starting the cryopreservation process will benefit the artificial insemination with frozen-thawed boar sperm products and can provide reasonable fertility outcomes. Key Words: Boar, Sperm, Freezability, Flow cytometry, New sperm parameter 台灣畜產種原知識庫 - https://agrkb.angrin.tlri.gov.tw