第三十七卷(2008)
76.褐色菜鴨BAC 基因庫之建構
廖仁寶 黃文瑛 鄭裕信 吳明哲
行政院農業委員會畜產試驗所
本研究目的在以大片段DNA 方式建構基因庫，除可保存珍貴基因外，並可提供後續基因體學研究之用。本研究已初步建立褐色菜鴨大分子量DNA 分離與大片段部分分切DNA 分離和純化技術平台，並建立以BAC 建構基因庫的核心技術，累計兩次所建構之基因庫，平均插入片段大小分別為34.0 kb 與48.9 kb，而所保存的總株系數目約達11 萬個，其基因體覆蓋率為3.69 倍。
關鍵語：褐色菜鴨、BAC 基因庫、覆蓋率
CONSTRUCTION OF BAC LIBRARY OF BROWN TSAIYA
R. B. Liaw, W. Y. Huang, Y. S. Cheng and M. C. Wu
Livestock Research Institute, Council of Agriculture, Executive Yuan
The purpose of this study is to construct large-insert libraries to preserve valuable genes and to provide for the future genomic studies. In this study, the plateform of large-insert DNA separation and separation and purification of large partially digested DNA from Brown Tsaiya was primarily established. At the same time, the core technology of BAC library construction was set up. The average insert sizes were 34.0 and 48.9 kb, respectively, for two constructed genomic libraries. The total number of preserved clones reached to 110,000 and the genome coverage of the entire library was 3.69-fold.
Keywords: Brown Tsaiya, BAC Library, Coverage
  
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