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第三十八卷(2009)
53.台灣黃牛粒線體D環區序列變異分析
廖仁寶 陳若菁 李光復 林秀蓮 吳明哲
行政院農業委員會畜產試驗所
本研究之目的在於探討台灣黃牛恆春品系牛隻粒線體D環區序列之變異，並建構演化類緣關係圖與BLAST分析，藉以了解族群內之差異，及與其他牛種之相關性。在30頭台灣黃牛恆春品系牛隻之粒線體DNA D-loop區域序列解析的結果，其長度介於1037~1039 bp，經過多重序列之比對分析後，可得知此30條序列可分成主要的兩個叢集，一叢集由7條序列組成，另一叢集則由23條序列組成，且在此區域中含有28個變異點。其後再分別將兩叢集中之各一條序列與GenBank資料庫進行比對分析，得知與此兩條序列相似性最高的物種同為Bos taurus strain Korean Cheongwon yellow 3，但identity則分別為99%與97%。
關鍵語：台灣黃牛、粒線體D環區、變異點
SEQUENCE VARIATION ANALYSIS OF MITOCHONDRIAL D-LOOP REGION OF TAIWAN YELLOW CATTLE HENGCHEN LINE
R. B. Liaw, J. J. Chen, G. F. Lee, H. L. Lin and M. C. Wu
Livestock Research Institute, Council of Agriculture, Executive Yuan
Ten Tons Cow is designated as milk yield of 305-2X-ME greater than 10,000 kg for hot and humid environment in Taiwan. For breeding scheme of dairy cattle, selection on milk yield and quality associated with reproductive performance is essential to the hot and humid weather. In 2001, there were 32 test-off Ten Tons Cow (0.1% to 28,381 cows for milk test), and then number of cows up to 103 head in 2002, 144 head in 2003, 248 head in 2004, 326 head (0.8% to 37,820 cows for milk test) in 2005, and there were 327, 326, 552, 1172 head from 2006 to 2009. An increment of 37 times (1172/32) for the number of ten tons cows for the past nine years. From 2001 to 2009, age of test-off Ten Tons Cow were 52, 57, 50, 55, 55, 51, 53, 53 and 51 months old, respectively; along with number of farms of 25, 50, 66, 88, 93, 87, 90, 93 and 116. In addition, a total of 3009 cows’ milk DNA was used to identify the genotype of four reproductive genes such as BLAD, CITL, CVM and DUMPS. There were 2945, 3007, 2711 and 3007 head with normal genotype of reproductive genes. In 2009, 574 of 1172 Ten tons cows were genotyped with 15% of animals having the CV genotype, which the CV genotype was unfavorable to conception of cows during summer season. Application of genomic selection combined with selection of milk yield and quality would be feasible to improve reproductive performance in Ten tons cows.
Key Words: Dairy cattle, Selection, Gene
  
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