行事曆
上個月2024年 3月下個月
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
今天
 主選單
 出版刊物
 訪問統計

 隨機小語
除非人類能夠將愛心延伸到所有的生物上,否則人類將永遠無法找到和平

[史懷哲]
 隨機文章
約克夏完檢(英文) (1988)
杜洛克公豬合照(中文) (1142)
約克夏公豬合照(英文) (1110)
約克夏側面照 (720)
中國畜牧2012年六月號 (821)
 熱門連結網享


分頁目錄
1. 水鹿類源關係與基因多樣性分析
2. 台灣「高大型種豬」之評估研究
3. 台灣杜洛克豬新品種選育: 級進世代毛色與基因型頻率
4. 乳牛繁殖基因型對乳量乳質性狀表現之影響
5. 黑山羊基因多樣性分析
6. 種豬腳蹄結構性狀之檢測
7. 種豬與種土雞之產精能力之研究
8. 種雞生長性能及孵化率之場內檢定
9. 雞隻泌乳素基因與藾菢性之關聯性探討
10. 雞隻動情素接受體基因與母雞早熟性之關聯性探討
第三十九卷(2010)
46.水鹿類源關係與基因多樣性分析

廖仁寶 陳若菁 林秀蓮 康獻仁 王治華 程梅萍 吳明哲
行政院農業委員會畜產試驗所

應用鹿隻mtDNA cytb基因與D-loop區專一之引子對,進行32個水鹿樣品DNA之PCR增幅放大,純化產物後,進行序列解析,將最後所得之各30條序列分別建構類源關係演化圖,發現若以cytb基因序列分析時,水鹿族群可分成主要的兩群,一群由27條序列組成,另一群則由3條序列組成,但若以D-loop區序列進行比對分析時,則可得知水鹿族群可分成五群,由此顯示D-loop區序列的演化變異率較cytb基因為大。此外,以16組微衛星型遺傳標記分析32頭台灣水鹿之基因多樣性,發現其觀測異質度、期望異質度及多態性訊息量範圍分別為0–0.84、0–0.73及0–0.68,而其平均值則分別為0.32± 0.24、0.38 ±0.27及0.34 ± 0.25,可得知此群水鹿的基因多樣性屬於低度多態性資訊。

關鍵詞:水鹿、類源關係演化圖、基因多樣性。


PHYLOGENETIC AND GENETIC DIVERSITY ANALYSES OF SAMBAR DEER

R. B. Liaw, R. J. Chern, H. L. Lin, S. R. Kang, C. H. Wang, M. P. Cheng and M. C. Wu

Livestock Research Institute, Council of Agriculture, Executive Yuan

The primer sets specific to cytb gene and mitochondrial D-loop region of deer were designed and used to amplify 32 sambar deer DNA. After purification, the PCR products were sequenced by an automatic DNA sequencer. The sequences of cytb gene and D-loop region from 30 samples were used to construct phylogenetic trees, respectively. Based on cytb gene analysis, the phylogenetic tree had two clusters; one cluster was composed of 27 sequences, the other 3 sequences. Based on the mitochondrial D-loop sequence analysis, the 30 sequences were divided into five clusters. The result indicates that the evolution rate of D-loop region is faster than that of cytb gene. Besides, the genetic diversity analysis of 32 sambar deer was conducted by using 16 microsatellite markers. The values for observed heterozygosity (Ho), expected heterozygosity (He), and polymorphism information content (PIC) among all loci were in the range of 0–0.84、0–0.73 and 0–0.68, respectively. Furthermore, the mean values for Ho, He, and PIC were 0.32±0.24、0.38±0.27 and 0.34±0.25, respectively. The result indicates that the genetic diversity of this sambar deer population is low.

Key Words: Sambar deer, Phylogenetic tree, Genetic diversity.

友善列印轉寄好友 輸出為 PDF 格式

  << List | Home | Top >> 第三十九卷(2010) © Copyright 2024 shuYing & 台灣畜產種原知識庫
 visiter

 本頁 QR Code

 搜尋

進階搜尋

 電子相簿
中央畜產會201409期D0197-04拍賣照片

 隨機好書

 夥伴網站

http://www.angrin.tlri.gov.tw

http://www.naif.org.tw/

http://www.coa.gov.tw

http://www.tlrihc.gov.tw/

http://minipigs.angrin.tlri.gov.tw