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第四十一卷(2012)
純種豬品系選育及品系雜交之基因標記研究
吳明哲(1)、林德育(1)、林秀蓮(1)、廖仁寶(1)、陳美如(1)、賴永裕(1)、顏念慈(1)、蔡秀容(1)、 林鴻霖(2)、王受鎔(2)、林欽德(2)、葉昇炎(3)、陳中興(3)
(1)行政院農業委員會畜產試驗所 (2)中央畜產會種豬檢定站 (3)行政院農業委員會畜牧處
純種豬藍瑞斯(L)、約克夏(Y)與杜洛克(D)品種，在肉豬生產體系中提供LYD三品種雜交優勢，進而有終端公豬品種─D品種之品系選育要求。本研究應用豬基因體組之第19號XY性染色體上的基因標記14個：SWR17、SW1325、SW1411、SW2156、SW980、SW2470、SW1522、SW1426、SY11、SO117、SW1943、SN218、SW2059、SW2588來建立純種豬個體的品系雜度%及品系基因差度%。因此，本研究自2010年1月至2012年5月間送檢仔豬群，計20期的生長性能檢定完檢豬隻取DNA樣本進行14個基因標記檢測。選定XY染色體上區SW1325的基因序列長(bp)為品系基因型，D品種(1,223頭)有11個品系(121~165bp)，其中121、151、155、163bp等四個主要品系；L品種(643頭)有13個品系(119~161bp)，其中143、151、153、155、157bp等五個主要品系；Y品種(241頭)有13個品系(115~163bp)，其中149、153、155、161bp等四個主要品系。當XY性染色體上的14個基因標記個別基因型均為純合型的個體數之百分比作為純品系選育程度，在D、L、Y品種分別已有7.4%(91/1223)、3.1%(20/643)、10.4%(25/241)選育為純品系；而D、L、Y品種的品系雜交個體之品系雜度%平均分別為22.3%、28.5%、25.7%；D、L、Y品種內品系雜交個體基因差度%(序列長)平均分別為1.37%(+27bp)、1.35%(+26bp)、1.05%(+20bp)。應用豬基因體組之第19號XY性染色體上的基因標記14個進行純品系選育可行，並有助於種豬場建置其原種族群(GP)與原原種族群(GGP)。
關鍵語：基因、選育、品系
GENETIC MARKERS FOR LINE SELECTION AND CROSSING OF PIG PUREBREDS
M. C. Wu(1), D. Y. Lin(1) , H. L. Lin(1), R. B. Liao(1), M. L. Chen(1), Y. Y. Lai(1), N. T. Yen(1), H. L. Tsai(1), H. R. Lin(2), H. L. Wang(2), C. T. Lin(2), S. Y. Yip(3) and C. S. Chen(3)
(1)Livestock Research Institute, Council of Agriculture, Executive Yuan; (2)Pig Performance Test Station, National Animal Industry Foundation; (3)Animal Industry Division, Council of Agriculture, Executive Yuan
Breeding scheme of Landrace(L), Yorkshire(Y) and Duroc(D) for production of three-way cross LYD hog with terminal sire breed of D is a practice of hybrid advantage. A total of 14 genetic markers on sexual chromosomal XY including of SWR17, SW1325, SW1411, SW2156, SW980, SW2470, SW1522, SW1426, SY11, SO117, SW1943, SN218, SW2059, SW2588 was used to conduct the measurements of pure line and gene sequence variation of pig purebreds. Piglets of 20 classes from 2010 January to 2012 May at Pig Performance Test Station were collected their DNA samples during growth performance test. Among of 14 genetic markers, the length of DNA fragment (bp) of SW1325 was designated as the genetic marker of each family line. There were 11 lines varied with 121~165bp in D breed (1,223 head) with a major of four lines on 121, 151, 155, 163bp; L breed (643 head) had 13 lines in 119~161bp of length with a major of five lines on 143, 151, 153, 155, 157bp; and Y breed (241 head) had 13 lines in 115~163bp of length with a major of four lines on 149, 153, 155, 161bp. When an individual having homo-genotypes on all of 14 markers is designated as a pure line animal, then there were 7.4%(91/1223), 3.1%(20/643), 10.4%(25/241) of pure lines in D, L, Y purebreds. Line crossing of D, L, Y purebreds were measured with percentage of hetero-genotypes and there were in average of 22.3%, 28.5%, 25.7%, along with the variation on gene sequence length of 1.37%(+27bp), 1.35%(+26bp), 1.05%(+20bp) in average. In this study, application of 14 genetic markers on sexual chromosomal XY to the line selection of pig purebreds is feasible and is possible to establish a GP and/or GGP breeding stock herd by genomic selection in pig purebreds.
Key Words: Gene, Selection, Line
  
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出類拔萃的人都有一個共通點：使命感。
[金克拉] |
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