第四十三卷(2014)
畜產試驗所高產蛋土雞品系CM之微衛星遺傳標記多樣性分析
林德育(1) 曾淑貞(2) 林義福(1) 林秀蓮(1) 張秀鑾(3) 吳明哲(1)
(1)行政院農業委員會畜產試驗所 (2)中華醫事科技大學 (3)國立屏東科技大學
畜產試驗所高產蛋品系CM係以畜試土雞台畜一號近親品系L12公雞與配商用褐色蛋雞母雞雜交一代為基礎族群,進行6個世代自交與產蛋數選育所育成的品系。為評估高產蛋品系CM選育族群的遺傳變異,本試驗利用FAO(2004)建議使用的23組雞微衛星標記組,以及LEI0258微衛星標記組,共24組微衛星標記組分析該族群候選種雞80隻之個體DNA。所有微衛星標記組皆有檢測出多態型的基因型。共檢測到80個對偶基因,平均每個基因座具有3.3個對偶基因(1~7個),其期望異質度介於0.013到0.701,平均為0.51,觀測異質度介於0到0.738,平均為0.49,而多態性訊息含量平均為0.44。在選用的24組微衛星標記組中有11組呈現高度多態性資訊(PIC ≥ 0.5),有10組呈現中度多態性資訊(0.5> PIC ≥ 0.25),3組呈現低度多態性資訊(PIC < 0.25)。本結果提供此選育族群遺傳變異之基本分子資訊。
關鍵語:土雞、遺傳多樣性、微衞星標記
GENETIC DIVERSITY ANALYSIS OF HIGH EGG PRODUCTION LINE CM NATIVE CHICKEN IN LRI-COA BY MICROSATELLITE MARKERS
D. Y. Lin(1), S. J. Tzeng(2), Y. F. Lin(1), H. L. Lin(1), H. L. Chang(3) and M. C. Wu(1)
(1)Livestock Research Institute(LRI), Council of Agriculture (2)Chung Hwa University of Medical Technology (3)National Pingtung University of Science and Technology
LRI high egg production line CM native chicken, which was selected for numbers of egg based on the F1 population crossed by the cocks of inbreeding line L12 native chicken of Council of Agriculture Livestock Research LRI and hens of commercial brown layer for six generation self-cross. To assess the genetic variability of high egg production line CM breeding populations, we use a set of 24 microsatellite markers to analyze 80 candidate bred chickens from this flock. All of the microsatellites were polymorphic. The average allelic number was 3.3, ranged from 2 to 7 per locus. The expected heterozygosity ranged from 0.013 to 0.701, and the average expected heterozygosity was 0.51±0.19 (mean ±SD). The observed heterozygosity of the population ranged from 0 to 0.738, and the average observed heterozygosity was 0.49±0.22. The estimated average polymorphic information content (PIC) was 0.44±0.18. In 24 markers, eleven markers were highly informative with polymorphism information content (PIC ≥0. 50), ten markers were reasonably informative (0.5> PIC≥ 0.25) and the other three markers were slightly informative (PIC < 0.25). These results could be provided basic molecular information for the research on the germplasm characteristics of high egg production line CM native chicken in LRI-COA.
Key Words: Native chicken, Genetic diversity, Microsatellite marker
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