第四十四卷(2015)
畜產試驗所白水牛群之微衛星遺傳標記多樣性分析
林德育(1) 曾淑貞(2) 莊璧華(1) 賴永裕(1) 蘇安國(1) 吳明哲(1)
(1)行政院農業委員會畜產試驗所 (2)中華醫事科技大學
為探討行政院農業委員會畜產試驗所花蓮種畜繁殖場台灣水牛保種族群中白水牛群之遺傳多樣性。本試驗利用 FAO(2004)建議使用的 12 組水牛微衛星標記分析該場全場 9 頭 白水牛個體之 DNA，其中除 CSSM045 與 CSSM070 微衛星標記所檢測的基因型在所有檢測個體皆為單型外，其它 10 組微衛星標記皆有多態型的基因型。共檢測到 32 個等位 基因(alleles)，平均每個基因座其有 2.7 個等位基因，其觀測異質度介於 0 到 0.778，平均為 0.389，期望異質度介於 0 到 0.699，平均為 0.483，而多態性訊息含量介於 0 到 0.611，平均為 0.404。在本試驗選用的 12 組微衛星標記組中，除 2 組微衛星標記在所有檢測樣品僅出現單一等位基因型外，有5 組呈現高多態性資訊(PIC 孟 0.5)，有 5 組 呈現中多態性資訊(0.5 > PIC 孟 0.25)，而平均期望異質度與平均觀測異質度均高於 0.25， 顯示此保種族群仍保有相當程度的遺傳多樣性，惟 CSSM045 與 CSSM070 標記分析所有個體皆呈現單一基因型，可能是當初引種的個體就僅有單一基因型，或在保種族群建立後喪失了其它基因型。因此，育種者應更加重視此白水牛群之遺傳多樣性的維護。
關鍵語:遺傳多樣性、微衛星標記、白水牛
GENETIC DIVERSITY ANALYSIS OF WHITE TAIWAN WATER BUFFALO HERD IN LRI-COA BY MICROSATELLITE MARKERS
D.Y. Lin(1), S. J. Tzeng(2), P. H. Chuang(1), Y. Y. Lai(1), A. K. Su(1) and M. C. Wu(1)
(1)Livestock Research Institute (LRI), Council of Agriculture (2)Chung Hwa University of Medical Technology
To study the genetic diversity of white water buffalo population in Taiwan Water Buffalo conservation herd of Hualien Animal Propagation Station of LRI. A set of 12 microsatellite markers recommended for water buffalo in FAO’s DADIS MoDAD programme were utilized to analyze all 9 animals to generate genotype data. Except CSSM045 and CSSM070, all the microsatellites were polymorphic with average allelic number 2.7, ranged from 1 to 4 alleles per locus. There were 32 alleles detected in total. The observed heterozygosity of the population ranged from 0 to 0.778, and the average of observed heterozygosity was 0.389. The expected heterozygosity ranged from 0 to 0.699, and the average of expected heterozygosity was 0.483. The estimated polymorphic information content (PIC) ranged from 0 to 0.611, and the average of PIC was 0.404. In 12 markers, five markers were highly informative with PIC more than 0.5 and two markers, CSSM045 and CSSM070, had only one allele detected. Therefore, geneticist should be aware of the genetic diversity of white water buffalo herd.
Key Words: Genetic diversity, Microsatellite marker, White Taiwan Water Buffalo
  
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