第四十七卷(2018)
126.畜試土雞品系L9母雞 Z 染色體上SNP基因型與產蛋性能的分析
林德育(1) 曾淑貞(2) 賴永裕(1) 林秀蓮(1) 洪哲明(1) 吳明哲(1)
(1)行政院農業委員會畜產試驗所 (2)中華醫事科技大學
為探討母雞Z染色體上SNP基因型與其產蛋性能之關聯性。本試驗利用雞Z染色體上131組SNP引子組檢測分析92隻畜試土雞品系L9母雞個體DNA，並將檢測出的SNP基因型與母雞產蛋性能進行關聯性分析。其中有82組SNP引子組所檢測出基因型在所有檢測個體皆為單型外，其它49組引子組皆有檢測出不同基因型。將母雞SNP基因型與其產蛋性能進行差異性分析，有3組引子組的不同基因型在母雞初產日齡有顯著差異(P<0.05)，分別有3組與5組引子組的不同基因型在母雞初產體重與蛋重有顯著差異(P<0.05)，有2組引子組的不同基因型在母雞達40週齡的產蛋數有顯著差異(P<0.05)，各有3組引子組所檢測出的不同基因型在母雞達40週齡的體重與平均蛋重有顯著差異(P<0.05)，而有4組引子組的不同基因型在母雞達280日齡的藾菢天數有顯著差異(P<0.05)。本結果提供此選育族群基因選種應用的候選遺傳標記之分子資訊。
關鍵語：雞、SNP、產蛋性能
Analysis of SNP genotypes on Z chromosome and laying performance in LRI-L9 hens
D. Y. Lin(1), S. J. Tzeng(2), Y. Y. Lai(1), H. L. Lin(1), C. M. Hung(1) and M. C. Wu(1)
(1)Livestock Research Institute(LRI), Council of Agriculture (2)Chung Hwa University of Medical Technology
To study the relationship between SNP genotypes on the Z chromosome of hens and their laying performance. In this experiment, 131 SNP primers on chicken Z chromosome were used to detect the individual DNA of 92 hens in the LRI-L9 selection flock, and the detected SNP genotypes were analyzed the correlation with laying performance of hens. Among them, 82 SNP primer kits detected genotypes in all tested individuals were single type, and other 49 primer kits detected different genotypes. Differences in hens' SNP genotypes and their laying performance were analyzed. We found that it was significant differences in the age at first egg of hens with different genotypes in 3 SNP primer kits (P<0.05), and different genotypes in 3 and 5 SNP primer kits were significant difference in the body weight and egg weight at 1st egg of hens, respectively (P<0.05). Different genotypes of hens detected by two SNP primer kits were significant difference in number of eggs laid up to 40 weeks of age. It was found different genotypes in each 3 SNP primer kits were significant difference in the body weight and egg weight at 40 weeks of age of hens, respectively (P<0.05). while the hens with different genotypes detected by 4 SNP primer kits had significant differences in broody days up to 40 weeks of age (P<0.05). This result provides molecular information on candidate genetic markers for this selection flock.
Key Words: Chicken, Single nucleotide polymorphism, Laying performance
  
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