第四十八卷(2019)
118.畜試土雞選育族群之家禽白血病 J 病毒監測
林德育 (1) 曾淑貞 (2) 洪哲明 (1) 蔡銘洋 (1) 賴永裕 (1) 朱家德 (1) 吳明哲 (1)
(1) 行政院農業委員會畜產試驗所 (2) 中華醫事科技大學
家禽白血病(avian leucosis, AL)是由家禽白血病病毒(avian leucosis virus, ALV)引 起，其中的 J 亞群(subgroup J ALV﹔ALV-J)於 1989 年出現，造成養雞業者的嚴重 損失。為了解行政院農業委員會畜產試驗所畜試土雞選育族群種雞群是否成染家禽白 血病 J 病毒，於本(2019)年進行全場選育族群候選種雞家禽白血病 J 病毒篩檢。以 含抗凝J EDTA-K3 之採血器採集雞隻翼靜脈 2 毫升全血，以核酸萃取試J進行核酸 萃取後，進行 PCR(primer H5/H7)檢測家禽白血病 J 病毒。檢測 4 個畜試土雞近親 品率的候選種雞，包括 263 隻品率 L7、202 隻品率 L9、212 隻品率 L11 及 191 隻品 率 L12，共 868 隻。檢測結果在所有送檢樣品皆呈陰性反應，顯示該選育族群為一家 禽白血病 J 病毒清淨族群。
關鍵語：家禽白血病、家禽白血病 J 病毒、雞、監測
Avian leucosis J-virus monitoring in the selection population of LRI native inbreeding lines
D. Y. Lin(1), S. J. Tzeng(2), C. M. Hung(1), M. Y. Tsai(1), Y. Y. Lai(1), C. T. Chu(1) and M. C. Wu(1)
(1) Livestock Research Institute (LRI), Council of Agriculture (2) Chung Hwa University of Medical Technology
Avian leucosis is caused by avian leucosis viruses (ALVs) are prevalent in the poultry industry worldwide and cause severe economic losses. The subgroup J of ALV (ALV-J) has emerged as an important pathogen of meat-type chickens since 1989 and causes serious economic losses in poultry industry. In order to monitor ALV-J disease in the selection population of LRI native inbreeding lines, we collected blood samples of candidate breeder chicken by the blood collection device with anticoagulant EDTA-K3 in 2019. The DNAs of blood samples were extracted with nucleic acid extraction reagent and the primer kits (H5/H7) was used for ALV-J PCR detection. Four inbreeding lines of LRI native chicken were monitored in this program. Totally 868 blood samples of candidate breeder chickens including 263 Line 7, 202 Line 9, 212 Line11 and 191 L12. All of the samples were ALV-J negative. It shows that the selection population of LRI native inbreeding lines is an avian leukemia J virus free population.
Key Words: Avian leucosis, ALV-J, Chicken, Monitoring
  
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