第四十八卷(2019)
121.檢定站種公豬精子成熟度與飼料效率之關聯性
朱巧倩 (1) 王受鎔 (2) 謝佳容 (1) 林秀蓮 (1) 郭廷雍 (1) 朱家德 (1) 吳明哲 (1)
(1) 行政院農業委員會畜產試驗所 (2) 財團法人中央畜產會
粒線體是精子泳動及授精能力的關鍵因子，未成熟精子的粒線體潛能較成熟精子的粒 線體潛能為低，精子代謝所需要的能量由粒線體提供，因此粒線體的完整度為測定精 子品質的重要依據。本研究測定之年青種公豬為財團法人中央畜產會種豬性能檢定站 2016 年至 2018 年共 24 期完檢之杜洛克（n = 1,061）、藍瑞斯（n = 420）及約克夏（n = 161）等 3 個品種計 1,642 頭種公豬。種公豬於拍賣前 20 天採集精液，採集之新鮮 精液儲存於 17℃保溫攜回實驗室測定精液濃度，運用流式細胞儀配合可與粒線體結 合的專一性染劑，染劑會依精子粒線體膜電位的不同呈現不同螢光，每頭公豬精液至 少測定 5,000 隻精子之粒線體完整度，作為判別年青公豬產精能力指標，藉此評估年 輕種公豬精子的成熟度，並比較精子成熟度與飼料效率之關聯性，以提供送檢豬場作 為評估場內種豬還育依據。
關鍵語：種豬、精子、粒線體完整性
Correlation between the maturation of sperm and the feed conversion ratio in performance-testing boars
C. C. Chu(1), S. J. Wang(2), C. J. Hsieh(1), H. L. Lin(1), T. Y. Kuo(1), C. T. Chu(1) and M. C. Wu(1)
(1) Livestock Research Institute, Council of Agriculture, Executive Yuan (2) National Animal Industry Foundation
Mitochondria play an important role in the motility and fertility of sperm by providing energy for metabolism. Therefore the mitochondria integrity is a vital criterion for grading the quality of sperm. This study was conducted to monitor the 24 batches of performance- testing boars including 1,061 Duroc, 420 Landrace and 161 Yorkshire breeds from 2016 to 2018. The semen was collected from boars by 20 days before the auction and stored at 17ºC. In consequence, the semen was analyzed by the flow cytometry, at least 5,000 sperms were checked for the integrity of mitochondria to distinguish the sperm productive capability of young boars. Eventually, the correlation between the maturation of sperm and the feed conversion ratio was clarified and it could be the reference for the selection of breeding pigs.
Key Words: Breeding pig, Sperm, Mitochondrial integrity
  
|
|
|
