第五十卷(2021)
43. 即時聚合酶鏈鎖反應檢測雞隻 OCX-32 基因點突變初探
朱家德(1) 林德育(1) 賴永裕(1) 吳明哲(1) 蕭振文(1) 張秀鑾(2)
(1)行政院農業委員會畜產試驗所 (2)國立屏束科技大學
種雞蛋蛋殼厚度（eggshell thickness, EST）與種蛋孵化率（hatchability, %）呈顯著正相關（r = 0.30，P < 0.05）。 Ovocalyxin-32（OCX-32）蛋白質於蛋殼形成期的子宮液內其有高度表現量，被視為影響蛋殼品質性狀之數量性狀基因座之一。本試驗運用即時聚合酶連鎖反應（real-time PCR）建立 OCX-32 基因 A7061C 突變點多態性基因型檢測平台，分析紅羽土雞、烏骨雞及龍門蛋雞 OCX-32-A7061C 基因型頻率分布。初步結果顯示紅羽土雞、烏骨雞及龍門蛋雞樣本中，C（cytosin）交替基因頻率分別為 0.67、0.92 及 1.00。未來擬對 OCX-32 基因 A7061C 點突變基因型與種雞蛋蛋殼厚度及種蛋孵化率進行統計分析，期藉此明暸 OCX-32 基因 A7061C 點突變與種雞蛋蛋殼厚度及種蛋孵化率之關聯性。
關鍵語：Ovocalyxin-32 基因、雞、蛋殼厚度、孵化率、即時聚合酶鏈鎖反應
Preliminary study on Genotypic analysis of single nucleotide polymorphism on OCX-32 gene in chicken by real-time PCR platform
C. T. Chu(1), D. Y. Lin(1), Y. Y. Lai(1), M. C. Wu(1), J. W. Shiau(1) and H. L. Chang(2)
(1) Livestock Research Institute, Council of Agriculture
(2)National Pingtung University of Science and Technology
Eggshell quality has a significantly positive correlation with hatchability (r = 0.30, P < 0.05). Ovocalyxin-32 (OCX-32), a 32-kDa protein, is present at high levels in the uterine fluid during the terminal phase of eggshell formation and is identified as a potential candidate gene influencing the eggshell traits. This study is to realize the genotypic frequency of single nucleotide polymorphism (A7061C) on OCX-32 gene by real-time PCR platform in red feather native chicken, black feather native Chicken, and Lohmann layer (50 samples per breed). The results illustrates that allele C frequencies were 0.67, 0.92, and 1.00 in red feather native chicken, black feather native chicken, and Lohmann layer. Further, we will proceed with the statistical analyses to identify the polymorphism of OCX-32 gene associated with the EST and hatchability in different breeds of chicken.
Key Words: Ovocalyxin-32 gene, Chicken, Eggshell thickness, Hatchability, Real-time PCR
  
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