第五十卷(2021)
115.臺灣登錄山羊微衛星遺傳標記基因多樣性分析
謝佳容(1) 曾楷扉(2) 陳若菁(1) 蕭振文(1)
(1)行政院農業委員會畜產試驗所遺傳育種組 (2)行政院農業委員會畜產試驗所高雄種畜繁殖場
本研究將藉由微衛星遺傳標記了解目前臺灣登錄山羊族群的基因多樣性，首先以南部 某羊場登錄種羊為標的，其在養登錄山羊包括台灣黑山羊恆春品系 74 頭、墾丁山羊 36 頭、波爾山羊 23 頭、努比亞山羊 39 頭及阿爾拜因山羊 8 頭，共 180 頭。以國際農糧組織建置之山羊微衛星標記檢測平台所提供之引子，藉由 MAF065、MCM527、TCRVB6、SRCRSP9、OarFCB48、CSRD247、SRCRSP23、INRA063、OarAE54、ILSTS005、SPS113、SRCRSP8、ILSTS087、INRABERN172、ILSTS029 與 DRBP1 等 16 個微衛星標記分析此場羊隻的遺傳多樣性。結果顯示此 16 組微衛星標記共檢測到 92 個對偶基因，平均每個基因座其有 5.8 個對偶基因。並且其期望異質度介於 0.234 至 0.896，平均為 0.554，觀測異質度則介於 0.145 至 0.945，平均為 0.537，多態性訊息含量介於 0.214 至 0.964，其平均為 0.620，16 組微衛星標記皆其有高多態性訊息含量（PIC > 0.5)。
關鍵語：山羊、基因多樣性、微衛星標記
The genetic diversity analysis with microsatellite markers of Taiwan registered goats
C. J. Hsieh(1), K. F. Tseng(2), J. C. Chen(1) and J. W. Shiau(1)
(1)Division of Breeding and Genetic, Livestock Research Institute, COA
(2)Kaohsiung Animal Propagation Station, Livestock Research Institute, COA
This study was conducted to elucidate the gene diversity of registered goats in Taiwan and start with the goat breeding farm in the south of Taiwan initially. Totally 180 heads of goats were involved in this experiment which included 74 Taiwan black goat Hengchun line, 36 Kenting goats, 23 Boer goats, 39 Nubian goats, and 8 Alpine goats. Based on the well-approved microsatellite markers from FAO, 16 markers such as MAF065, MCM527, TCRVB6, SRCRSP9, OarFCB48, CSRD247, SRCRSP23, INRA063, OarAE54, ILSTS005, SPS113, SRCRSP8, ILSTS087, INRABERN172, ILSTS029, and DRBP1 were applied to view the gene diversity of goats on this farm. The results showed that the average values of expected heterozygosity were 0.234 to 0.896 and had a mean value of 0.554. Meanwhile, the averages of observed heterozygosity ranged from 0.145 to 0.945 and had a mean value of 0.537. The polymorphic information content (PIC) was estimated with a mean of 0.620 and ranged from 0.214 to 0.964. All 16 microsatellite markers were highly informative (PIC > 0.5).
Key Words: Goat, Genetic diversity, Microsatellite makers
  
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