第三十四卷(2005)
結合程式語言讀寫格式編輯DNA條碼
黃鈺嘉 顏念慈 賴永裕 林德育 吳明哲
行政院農業委員會畜產試驗所
程式語言讀寫格式具有讀取/儲存短整數、長整數、字串、二進位、八進位、十六進位等資料格式功能，與DNA訊息結合編輯後，可作為DNA條碼的一部份。此類DNA條碼，不適合採用EAN-8或EAN-13等零售商使用的短位數一維條碼，因為短條碼本身無法提供足量的商品資訊，除非與後端的資料庫結合。當長位數的條碼作為DNA條碼使用時，DNA條碼本身即可作為產品的特性證明，如 EAN-128或二維條碼，使用者可以在掃讀後直接了解條碼儲藏的DNA資訊。本試驗以EAN-128 編輯雞與牛的單點核酸變異(SNP)遺傳標記條碼，同時嘗試將不同豬品種的粒腺體 D-loop 區段以PDF417二維條碼編輯列印。利用識別碼加上程式語言讀取/儲存格式、識別碼加上遺傳標記名稱與遺傳型，或識別碼加上DNA序列資料等組合式條碼編輯方法進行編碼。由於豬的粒腺體D-loop 區段超過1000個以上的核酸，除以十六進位編輯方法外，亦可將整段序列的冗長共同區，以跳格的方式略去編輯，使條碼更為簡潔。DNA條碼的功能除了其他學者提過的保密與防偽功能(基因身份證)外，本試驗嘗試提供DNA條碼的另一種用途。
關鍵語：DNA條碼、單點核酸變異、核酸序列
DNA BARCODE EDITING WITH FORMAT STRING OF COMPUTER LANGUAGES
Y. C. Huang, N. T. Yen, Y. Y. Lai, D. Y. Lin and M. C. Wu
Livestock Research Institute, Council of Agriculture
Format string of computer languages can deal with short integer, long integer, character strings, octal Constants and hexadecimal constants. Editing DNA barcode with the format string can provide understandable message of product without connection of database. Although the major retail barcode types are "short" barcodes (for example, EAN-8, EAN-13), the connected database can provide enough information for business management. Using larger barcode zones for DNA barcodes can approach certification for bio-products by its own stored information. Sets of single nucleotide polymorphism of chicken and cattle, and nucleotide sequences of D-loop region in mitochondrial DNA of Sus scrofa domestica were edited to EAN-128 or PDF417 2D barcode with the format strings of computer language in this study. Application Identifiers (AI) and the format string data, AI and the SNP data, or AI and the nucleotide sequence data were tested. Because the D-loop region has more than 1000 nucleotide acid, hexadecimal constants or skip space format were used to simplify the storage of nucleotide sequence information. In addition of preventing imitation and protection secrete data (DNA ID), DNA barcode with the format string can provide information for traceability and quality control.
Key Words: DNA barcode, Single nucleotide polymorphism, Nucleotide sequence
  
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