第三十五卷(2006)
34.肉雞飼料用乳酸菌株之篩選分離
陳若菁(1)、沈韶儀(1)、黃建榕(1)、林德育(1)、陳椒華(2)
(1)行政院農業委員會畜產試驗所 (2)嘉南藥理科技大學生物科技系
將1、2、4、7及28日齡的雞隻糞便培養在MRS培養基上，挑選631個單一菌落，經凝乳反應 (curdling reaction)、革蘭氏染色 (Gram stain) 及觸酶反應 (catalast test) 等特性試驗之初步篩選後，得到177株菌株。再以16S rDNA序列分析進行菌種鑑定，共鑑定出46株菌，分別屬於Uncultured bacterium、Enterococcus faecalis、Lactobacillus johnsonii、Lactobacillus reuteri及Lactobacillus salivarius。對鑑定出之6株乳酸菌株 (Lactobacillus) 測定其產酸能力、生長速率及對酸與0.3%膽鹽的耐受性，其中以Lactobacillus salivarius 28DM-68的產酸能力最好，Lactobacillus johnsonii 4DM-45的耐酸及耐膽鹽能力較佳。大量培養這兩株菌後利用低溫冷凍乾燥方法，製成乾燥菌粉，其活菌存活率分別為10%及53%，將作為後續之肉雞飼料試驗的添加劑。
關鍵語：乳酸菌、益生菌、肉雞、篩選
ISOLATION OF POTENT LACTOBACILLUS STRAINS AS PROBIOTICS FOR THE BROILERS
J. C. Chen(1), S. Y. Sheen(1), C. J. Huang(1), D. Y. Lin(1) and J. H. Chen(2)
(1)Livestock Research Institute, Council of Agriculture, Executive Yuan
(2)Chia-Nan University of Pharmacy and Science
The feces from 1, 2, 4, 7 and 28 days old chickens are sampled and cultured on the MRS agar. Screening of the 631 single colonies by curdling reaction, Gram stain test and catalast test, we get 177 strains of lactic acid bacteria (LAB). These LABs are Uncultured bacteria, Enterococcus faecalis, Lactobacillus johnsonii, Lactobacillus reuteri and Lactobacillus salivarius as identified by the 16S rDNA sequencing. The six identified Lactobacillus strains are further studied for their acid productivities, growth rates and tolerance for acid and bile salts. Among them, the Lactobacillus salivarius 28DM-68 has the best acid productivity and Lactobacillus johnsonii 4DM-45 shows good performance on the tolerance for acid and bile salts. The viability of the freeze-dried culture for Lactobacillus salivarius 28DM-68 and Lactobacillus johnsonii 4DM-45 are 10% and 53%, respectively.
Key Words: Lactobacillus, Probiotics, Broiler, Isolation
  
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