第四十九卷(2020)
35.種豬厭氧腸道菌培養體學分析之初探
陳佳萱(1) 劉忠憲(2) 楊杰(3) 吳明哲(1) 黃建勳(2)
(1)行政院農業委員會畜產試驗所 (2)財團法人食品工業發展研究所 (3)福昌種豬場
影響宿主腸道健康之關鍵微生物存在於厭氧環境中，不易自腸道分離，故腸道菌培養體學技術對於發展新世代益生菌相當重要。本試驗採集杜洛克種公豬新鮮糞便樣本，使用3種不同的厭氧培養條件進行厭氧腸道菌之分離培養，再以基質輔助雷射脫附游離飛行時間式質譜儀 （MALDI-TOF MS） 或16S rRNA基因定序，快速鑑別所得分離株。結果顯示，總計挑選近500個分離菌落，經過繼代培養、菌株純化和學名鑑別，最後得到20株不同菌種別之分離株，這些菌株主要分別為厚壁菌門、擬桿菌門、放線菌門、變形菌門與Synergistetes門，其中有四株無法定義出菌種學名，推測可能是從未被發現的新穎菌種，未來將利用全基因體序列進行更進一步的比對分析，並結合傳統多相分類技術，藉以完成新種的描述與發表。
關鍵語：豬腸道菌相、培養體學分析、微生物新種
Preliminary study of gut microbiota in breeding pig using microbial culturomics analyses
C. H. Chen(1), J. S. Liou(2), C. Yang(3), M. C. Wu(1) and C. H. Huang(2)
(1)Livestock Research Institute, Council of Agriculture, Executive Yuan (2)Bioresource Collection and Research Center, Food Industry Research and Development Institute (3)Formosa Breeding Pig Farm
The host intestinal health may be affected by some crucial microorganisms, which are existed in the anaerobic environment and are difficult to isolate. Therefore, microbial culturomics technique for anaerobic intestinal microbiota is of great importance to the development of next-generation probiotics. The gut isolates include facultative and strict anaerobes that were isolated from pig feces samples under three different culture conditions. Nearly 500 isolated colonies were identified using MALDI-TOF MS (matrix-assisted laser desorption ionization time-of-flight mass spectrometry) combined with 16S rRNA sequencing, and successfully cultured 20 different bacterial species, which were belonged to the members of Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria and Synergistetes respectively. Among them, 4 new species have been discovered. These new taxa will be characterized and described by taxonogenomics approaches for proposing the novel species in the future.
Key Words: Swine gut microbiota, Culturomics, Microbial novel species
  
|
|
|
.jpg)
