第四十九卷(2020)
84.畜試所藍殼烏骨雞之微衛星遺傳標記多態性分析
林德育(1) 曾淑貞(2) 劉曉龍(1) 賴永裕(1) 蔡銘洋(1) 洪哲明(1) 吳明哲(1)
(1)行政院農業委員會畜產試驗所 (2)中華醫事科技大學
為評估行政院農業委員會畜產試驗所藍殼蛋烏骨雞選育族群的遺傳變異，本試驗利用FAO(2010)建議使用的24組雞微衛星標記組分析117隻藍殼蛋烏骨雞第G4世代種雞個體DNA。其中除MCW0103、MCW0216及MCW0248微衛星標記所檢測的基因型在所有檢測個體皆為單型外，其它21組微衛星標記皆有多態型的基因型。共檢測到87個對偶基因，平均每個基因座具有3.5個對偶基因(1~13)；其期望異質度介於0到0.774，平均為0.422，觀測異質度介於0到0.778，平均為0.372，而多態性訊息含量介於0到0.735，平均為0.377(表26)。在選用的24組微衛星標記組中有10 組呈現高度多態性資訊(PIC ≥ 0.50)，有5組呈現中度多態性資訊(0.50> PIC ≥ 0.25)，9組呈現低度多態性資訊(PIC < 0.25)。本結果提供此選育族群遺傳變異之基本分子資訊。
關鍵語：烏骨雞、微衞星遺傳標記、多態性
Polymorphism analysis of LRI blue egg silkie chicken by microsatellite markers
D. Y. Lin(1), S. J. Tzeng(2), H. L. Liu(1), Y. Y. Lai(1), M. Y. Tsai(1) , C. M. Hung(1) and M. C. Wu(1)
(1)Livestock Research Institute(LRI), Council of Agriculture (2)Chung Hwa University of Medical Technology
In order to evaluate genetic variation of LRI blue egg silkie chicken flock. we use a set of 24 microsatellite markers recommended by FAO to analyze 117 candidate bred chickens from this flock. Except MCW0103, MCW0216 and MCW0248, all the microsatellites were polymorphic. The average allelic number 3.5, ranged from 1 to 13 per locus. The expected heterozygosity ranged from 0 to 0.774, and the average expected heterozygosity was 0.422. The observed heterozygosity of the population ranged from 0 to 0.778, and the average observed heterozygosity was 0.372. The polymorphic information content (PIC) ranged from 0 to 0.774, and the average PIC was 0.491. In 24 markers, 10 markers were highly informative with polymorphism information content (PIC ≥ 0.50), five markers were reasonably informative(0.5 > PIC ≥ 0.25) and the other nine markers were slightly informative (PIC < 0.25). These results could be provided basic molecular information for the research on the germplasm characteristics of the population.
Key Words: Silkie chicken, Microsatellite marker, Polymorphism
  
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