第四十九卷(2020)
85.畜試所藍殼烏骨雞選育族群之家禽白血病 J 病毒監測
林德育(1) 曾淑貞(2) 劉曉龍(1) 蔡銘洋(1) 賴永裕(1) 朱家德(1) 吳明哲(1)
(1)行政院農業委員會畜產試驗所 (2)中華醫事科技大學
家禽白血病 （avian leucosis, AL） 是由家禽白血病病毒 （avian leucosis virus, ALV） 引起，其中的 J 亞群 （subgroup J ALV；ALV-J） 於 1989 年出現，造成養雞業者的嚴重損失。為了解行政院農業委員會畜產試驗所藍殼烏骨雞選育族群種雞群是否感染家禽白血病 J 病毒，於本 （2020） 年進行全場選育族群315隻候選種雞之家禽白血病 J 病毒篩檢。以含抗凝劑 EDTA-K3之採血器採集雞隻翼靜脈 2 毫升全血，以核酸萃取試劑進行核酸萃取後，進行 PCR （primer H5/H7） 檢測家禽白血病 J 病毒。檢測結果在所有送檢樣品皆呈陰性反應，顯示該選育族群為一家禽白血病 J 病毒清淨族群。
關鍵語：烏骨雞、家禽白血病、家禽白血病J病毒、監測
Avian leucosis J-virus monitoring in the selection population of LRI blue egg silkie chicken
D. Y. Lin(1), S. J. Tzeng(2), H. L. Liu(1), M. Y. Tsai(1), Y. Y. Lai(1), C. T. Chu(1) and M. C. Wu(1)
(1)Livestock Research Institute (LRI), Council of Agriculture (2)Chung Hwa University of Medical Technology
Avian leucosis is caused by avian leucosis viruses (ALVs) are prevalent in the poultry industry worldwide and cause severe economic losses. The subgroup J of ALV (ALV-J) has emerged as an important pathogen of meat-type chickens since 1989 and causes serious economic losses in poultry industry. In order to monitor ALV-J disease in the selection population of LRI blue egg silkie chicken, we collected blood samples of candidate breeder chicken by the blood collection device with anticoagulant EDTA-K3 in 2020. The DNAs of 315 silkie chicken blood samples were extracted with nucleic acid extraction reagent and the primer kits (H5/H7) was used for ALV-J PCR detection. All of the detected samples were ALV-J negative. It shows that the selection population of LRI blue egg silkie chicken is an avian leukemia J virus free population.
Key Words: Silkie chicken, Avian leucosis, Avian leucosis J-virus(ALV-J), Monitoring
  
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