第五十二卷(2023)
42. 即時聚合酶鏈鎖反應檢測商用土雞與蛋雞 OC-116 基因點突變之基因型分析
朱家德(1) 林德育(1) 賴永裕(1) 吳明哲(1) 張秀鑾(2)
(1)農業部畜產試驗所 (2)國立屏東科技大學
種蛋孵化率是影響市場雛雞供給量與經濟效益的關鍵因素之一，而種蛋孵化率高低顯著受 蛋殼厚薄程度所影響。本試驗告在應用即時聚合酶鏈鎖反應之競爭性交替基因特異性聚合酶鏈鎖反應 （kompetitive allele-specific polymerase chain reaction, KASP）技術，發展我國影 響蛋殼厚度候選基因 （ovocleidin-116, OC-116） 之突變點多態性檢測法。隨機抽取商業紅 羽土雞、烏骨雞與龍門蛋雞各 50 隻進行 DNA 萃取，檢測 OC-116-C1336G 基因型頻率分布。初步結果顯示商業紅羽土雞、烏骨雞及龍門蛋雞的 C （cytosine） 交替基因頻率分別 為 0.85、0.2 及 1。未來，會持續收集商業紅羽土雞不同週齡種蛋孵化記錄，並進行 OC-116- C1336G 基因型與種蛋孵化率之關聯性分析，以釐清此基因型對性能表現的影響程度。
關鍵語：蛋殼厚度、孵化率、ovocleidin-116 基因、即時聚合酶鏈鎖反應
Genotypic analysis of single nucleotide polymorphism on OC-116 gene in commercial native chicken and layer by real-time PCR platform
C. T. Chu(1), D. Y. Lin(1), Y. Y. Lai(1), M. C. Wu(1) and H. L. Chang(2)
(1)Taiwan Livestock Research Institute (2)National Pingtung University of Science and Technology
The hatchability is one of the key factors affecting the supply of market chicks and economic benefits, and it is significantly influenced by the eggshell thickness. The aim of this study was to develop a genotyping method for SNP of ovocleidin-116 in commercial native chicken and layer by using real-time PCR technology labeled with kompetitive allele-specific polymerase chain reaction (KASP). Commercial red feather native chicken, silkie, and Lohmann layers were randomly sampled for DNA extraction and genotyping with 50 birds per breed. Preliminary results indicated that the allelic frequency of cytosine in commercial red feather native chicken, silkie, and Lohmann layers were 0.85, 0.2, and 1, respectively. The effect of OC-116-C1336G on eggshell thickness and hatchability will be further investigated to confirm the essential influence.
Key Words: Eggshell thickness, Hatchability, Ovocleidin-116 gene, Real-time PCR
  
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