第五十二卷(2023)
155. 乳牛活體採卵效能提升
曲鳳翔(1) 郭廷雍(1) 陳裕信(2) 李佳蓉(2)
(1)農業部畜產試驗所遺傳生理組 (2)農業部畜產試驗所畜產經營組
人工生殖技術應用旨在提高家畜經濟效益，在這些技術應用中，體外胚胎生產（IVEP）受到關注，其在畜牧產業中的應用不斷增加。全球牛胚移置數量近十年間成長 10 倍（20 萬∼ 200 萬／每年）其中以活體採卵配合胚體外生產（OPU-IVP）占大多數（2022 年已達 68% 以上），已成為改善牛隻繁殖育種效能及提升單位產量的重點研發方向。本研究的目的是在評估乳牛活體採卵（Ovum Pick Up）採集卵母細胞之效能，以直腸觸診方式固定卵巢利用超音波顯影探頭配合採卵探針，吸取卵巢濾泡中的卵子，這樣的操作過程需要嫻熟的技巧才能達到 60%以上的回收率。經過表面鍍層的取卵探針，能有效提升在超音波顯示螢幕上的顯影效果，相較傳統探針卵子平均可提升回收率 7%。
關鍵語：乳牛、活體採卵、胚體外生產
Improved Efficiency of Oocytes Collection from Dairy Cattle
F. H. Chu(1), T. Y. Guo(1), Y. S. Chen(2) and J. R. Li(2)
(1)Genetics and Physiology Division, Taiwan Livestock Research Institute, Ministry of Agriculture
(2)Livestock Management Division, Taiwan Livestock Research Institute, Ministry of Agriculture
The application of artificial reproductive technologies aims to improve the economic benefits of livestock. Among these technological applications, in vitro embryo production (IVEP) has attracted attention, and its application in the livestock industry continues to increase. The number of bovine embryo transfers worldwide has increased tenfold in the past decade (200,000 to 2 million / year), of which live egg collection combined with in vitro embryo production (OPU-IVP) accounts for the majority (reaching more than 68% in 2022), which has become The key research and development direction is to improve the efficiency of cattle breeding and increase unit output. The purpose of this study is to evaluate the effectiveness of Ovum Pick Up in collecting oocytes from dairy cows. The ovaries are fixed in the rectal veterinary examination method and the eggs in the ovarian follicles are aspirated using an ultrasonic imaging probe combined with an egg collection probe. In this way the operation process requires skilled skills to achieve a recovery rate of more than 60%. The surface- coated egg retrieval probe can effectively improve the development effect on the ultrasonic display screen, and can increase the recovery rate by an average of 7% compared with traditional probes.
Key Words: Dairy cattle, Ovum Pick Up, Embryo in vitro production
  
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