第五十二卷(2023)
163. 應用精子新穎參數於種公豬精子耐凍性之評選
郭廷雍(1) 林信宏(2) 李耶瑩(3) 楊卓真(3) 楊國泰(4) 許順貴(5)
(1)農業部畜產試驗所遺傳育種組 (2)農業部畜產試驗所南區分所 (3)國立嘉義大學動物科學系
(4)國立屏東科技大學動物科學與畜產系(5)屏東縣金山種豬場
研究顯示冷凍保存對公豬精子造成損害，應用新穎精子功能參數測定可用作篩選公豬個體是否其備精子耐凍性外亦可檢測出質量差的豬冷凍精液。因此本研究目的為應用新穎精子 功能參數於種公豬個體問精子耐凍性進行分析外並指出精子損傷的潛在指標，以評選繁殖 性能耐凍公豬、排除質差冷凍豬精及冷凍配方改善，試驗結果顯示不同公豬個體問冷凍傷害主要造成精子活動力及存活力的下降，在耐凍性不佳之個體公豬在精液解凍前後顯現精子活力下降 37.0% (P< 0.05)、前向運動性下降 22.1% (P< 0.05)、存活力下降 24.0% (P< 0.05)及精子頭帽完整性下降 8.3 % (P< 0.05)、精子 DNA 完整性下降 1.9%及氧化傷害增加 2%，顯示公豬間其精子耐凍能力存在個體差異。綜上所述，在進行精液冷凍保存前對耐凍性評估和精子耐凍評選將有利於使用冷凍解凍的公豬精液產品進行人工授精並可得到合理的生育成績。
關鍵語：公豬、精子、流式細胞儀、精子新穎參數
Application of novel sperm function in assessment of the freezability of boar ejaculates
T. Y. Kuo(1), H. H. Lin(2), Y. Y. Lee(3), C. C. Yang(3), K. T. Yang(4) and S. K. Hsu(5)
(1)) Genetic and physiology Division, Livestock Research Institute, M.A. (2)South region branch, Livestock Research Institute, M.A (3)Department of Animal Science, National Chiayi University (4)Department of Animal Science, National Pingtung University of Science & Technology (5)Jinshan Breeding farm, Ligang Township, Pingtung County, Taiwan
Damage to boar sperm from cryopreservation is known to result in reduced motility, viability and litter size. Novel sperm function assays were used to detect poor quality frozen porcine semen prior to use and thus improve AI success. The purpose of this study was using novel sperm function assessment to characterize cryopreserved boar spermatozoa. Low freezability boar showed about 37.0% (P < 0.05) motility decrease, 22.1% (P < 0.05) progressive motility decrease, 24.0% (P < 0.05) sperm viability decrease and 8.3 % (P < 0.05) acrosome integrity decrease, 1.9% intact DNA decrease and 2% of oxidative stress increased after frozen-thawed were observed. In summary, freezable ejaculates assessment and sperm selection before starting the cryopreservation process will benefit the artificial insemination with frozen-thawed boar sperm products and can provide reasonable fertility outcomes.
Key Words: Boar, Sperm, Freezability, Flow cytometry, New sperm parameter
  
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