第五十二卷(2023)
98. 豬 GPAT1 與 PRKAG 3 基因點突變多態性之基因型分析
朱家德(l) 林德育(l) 賴永裕(l) 吳明哲(l) 張秀鑾(2)
(l)農業部畜產試驗所 (2)國立屏東科技大學
肌內脂肪(IMF）含量為影響肌肉柔嫩度、多汁性、氣味及風味關鍵因子之一。文獻指出， GPAT1 (Glycerol-3-phosphate acyltransferase l） 與 PRKAG3 (protein kinase AMP-activated non-catalytic subunit gamma 3） 基因點突變顯著影響背最長肌 IMF 與 pH 值。本研究旨在 應用即時聚合酶鏈鎖反應技術，發展影響豬隻 IMF 含量之候選基因突變點多態性檢測法。 逢機選取 13 頭杜洛克、13 頭藍瑞斯、8 頭約克夏與 13 頭黑豬進行多態性位點基因型鑑定， 初步結果顯示：前述品種豬隻 GPATl-SfaNI 位點 CC 基因型頻率依序為 3l、l00、l00 與 62%﹔GPATl-BbvI 位點 CC 基因型則依序為 39、39、63 與 l00%。PRKAG3-A325G 位點 AA 基因型頻率分別為 46、23.0、75 與 23%。未來將近一步分析 GPATl 與 PRKAG3 基因 前述突變點與豬隻背最長肌 IMF 含量相聯性，釐清其影響程度。
關鍵語：豬、GPATl 基因、PRKAG3 基因、即時聚合酶鏈鎖反應
Genotypic analysis of single nucleotide polymorphism on GPATl and PRKAG3 genes in commercial pigs
C. T. Chu(l), D. Y. Lin(l), Y. Y. Lai(l), M. C. Wu(l) and H. L. Chang(2)
(l)Taiwan Livestock Research Institute
(2)National Pingtung University of Science and Technology
Intramuscular fat (IMF) content is one of the key factors affecting pork tenderness, juiciness, odor, and flavor. The literature indicated that GPATl (Glycerol-3-phosphate acyltransferase l) and PRKAG3 (protein kinase AMP-activated non-catalytic subunit gamma 3) genes significantly affect the IMF content and pH value of the longissimus dorsi muscle in pig. This study aims to develop a genotyping method in pig breeds using real-time PCR technology. Thirteen Duroc, thirteen Landrace, eight Yorkshire, and thirteen black pigs were randomly sampled for genotyping. Preliminary results showed that the CC genotype frequencies of the above breeds at the GPATl- SfaNI site were 3l, l00, l00, and 62%, respectively﹔ the GPATl-BbvI site were 39, 39, 63 and l00%, respectively. However, the AA genotype frequencies for PRKAG3-A325G were 46, 23, 75, and 23%, respectively. In the future, we will further analyze the correlation between the above GPATl and PRKAG3 gene mutations and the IMF content of pig longissimus dorsi muscle to clarify their impact.
Key Words: Pig, GPATl gene, PRKAG3 gene, Real-time PCR
  
|
|
|
