第五十二卷(2023)
112. 臺灣登錄山羊微衛星遺傳標記基因多樣性分析一努比亞山羊族群
謝佳容(1) 陳若菁(1) 林德育(1)
(1)農業部畜產試驗所遺傳生理組
本研究藉由微衛星遺傳標記了解目前臺灣登錄山羊族群的基因多樣性，以國際農糧組織建 置之山羊微衛星標記檢測平台中 MAF065、MCM527、TCRVB6、SRCRSP9、OarFCB48、 CSRD247、SRCRSP23、INRA063、OarAE54 與 DRBP1 等 10 個微衛星標記分析南部某種 羊場 135 頭努比亞山羊。其檢測結果顯示共有 152 個交替基因，平均每個基因座其有 7.6 個
交替基因。並且其期望異質度介於 0.546 至 0.776，平均為 0.668，觀測異質度則介於 0.541 至 0.807，平均為 0.673，多態性訊息含量介於 0.492 至 0.744，其平均為 0.624；在此 10 組 微衛星標記中，有 9 組具有高多態性訊息含量（PIC>0.5）。本研究結果顯示此 9 組微衛星 標記能有效偵測努比亞山羊族群之遺傳多樣性，可以此進一步了解其遺傳結構與親緣關係， 作為未來品種選育之參考。
關鍵語：努比亞山羊、遺傳多樣性、微衛星標記
The genetic diversity analysis with microsatellite markers of Taiwan registered goats-Nubian goat population
C. J. Hsieh(1), J. C. Chen(1) and D. Y. Lin(1)
(1)Genetics and Physiology Division, Taiwan Livestock Research Institute, Ministry of Agriculture
This study was conducted to elucidate the genetic diversity of registered goats in Taiwan and focused on the Nubian goat population from a breeding goat farm in southern Taiwan. A total of 135 heads of Nubian goats were involved in this study. Ten FAO-approved microsatellite markers, including MAF065, MCM527, TCRVB6, SRCRSP9, OarFCB48, CSRD247, SRCRSP23, INRA063,
OarAE54, and DRBP1 were applied to analyze the genetic diversity of Nubian goats. The results showed that the expected heterozygosity values were 0.546 to 0.776 and had a mean value of 0.668. The observed heterozygosity values ranged from 0.541 to 0.807 and had a mean value of 0.673. The polymorphic information content (PIC) was estimated with a mean of 0.624 and ranged from 0.492 to 0.744. Nine of 10 microsatellite markers indicated highly informative (PIC > 0.5). The results showed that based on these nine microsatellite markers, the genetic diversity of black goat populations could be monitored, and the genetic structure and phylogenetics would be analyzed in the future.
Key Words: Nubian goats, Genetic diversity, Microsatellite markers
  
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