第五十二卷(2023)
123. 不同超音波導引探頭應用於淘汰牛隻體內撿卵之效果比較
郭廷雍(1) 曲鳳翔(1) 林信宏(2) 李佳馨(3) 李佳蓉(4) 梁世祥(4) 范耕榛(4) 楊鎮榮(1)
(1)農業部畜產試驗所 遺傳生理組 (2)農業部畜產試驗所 南區分所
(3)農業部畜產試驗所北區分所 (4)農業部畜產試驗所 畜產經營組
乳牛產業可以利用體內取卵法搭配體外胚生產法（OPU-IVEP）產製可移置胚，本法對於最 優良的牛群而言其備短短幾年內能迅速擴增及改良後代牛群最快速的優勢，即加速其高遺 傳優勢的潛力母牛和優異公牛中獲得後代的能力以實現更快的遺傳進展，並且解決了可移 置胚數量受限的瓶頸並讓遺傳優勢等級較高的胚胎的移植比例更高。OPU 系統由 3 個主 要組件組成，帶有適當探頭的超音波掃描儀、抽吸幫浦和連接到卵母細胞收集管的針引導 系統，讓牛隻體內取卵得以實現，本研究之目的為比較法式及日式超音波探頭及其針頭形 式應用於淘汰牛隻體內撿卵之效果，結果顯示卵子回收率在法式及日式探頭分別為 45%及 54%，回收卵子之卵丘細胞完整率為 75%及 85%及每頭牛隻平均約收集 3.5 顆卵子，綜上 所述，有鑑於其備卵丘細胞完整性評估對卵母細胞（COC）成熟和未來發育能力的重要性， 必須針對給定象統評估抽吸程序對 COC 造成的任何損害，以便採取預防措施以提高相關 卵子回收率及減少對卵丘細胞完整性的破壞。
關鍵語：體內取卵、牛、卵子、回收率
Comparison of the effects using different ultrasonic guidance probes on oocyte collection in culled cows
T. Y. Kuo(1), F. H. Chu(1), H. H. Lin(2), C. X. Lee(3), C. R. Lee(4), S. S. Liang(4), G. J. Fan(4)
and C. J. Yang(1)
(1)Genetic and Physiology Division, Livestock Research Institute, M.A.
(2)South region branch, Livestock Research Institute, M.A
(3)Nouth region branch, Livestock Research Institute, M.A
(4)Livestock Management Division, Livestock Research Institute, M.A.
An OPU system consists of 3 ma吋or components: an ultrasonographic scanner with an appropriate transducer (probe), an aspiration pump, and a needle guidance system connected to an oocyte collecting tube. The aim of this study was the comparison of the effects using different ultrasonic guidance probes and needles on oocyte collection in culled cows. The result shows that oocytes recovery rate guiding by France and Japanese probe were 45% and 54%. Rate of recovery oocytes with intact cumulus cell were 75%及 85%. Averagely, about 3.5 of oocytes were collected on each culled cow. In summary, given the importance of an intact cumulus cell investment for oocyte maturation and future developmental capacity, any damage to the COC caused by the aspiration procedure has to be assessed for a given system so that preventive procedures can be taken.
Key Words: Ovum picks up, Bovine, Oocyte, Recovery rate
  
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